Differential expression of oxidative stress and inflammation related genes in peripheral blood mononuclear cells in response to a low-calorie diet: a nutrigenomics study.

Abstract	Nutrigenomics is a new application of omics technologies in nutritional science. Nutrigenomics aims to identify molecular markers of diet-related diseases and mechanisms of interindividual variability in response to food. The aim of this study was to evaluate peripheral blood mononuclear cells (PBMC) as a model system and readily available source of RNA to discern gene expression signatures in relation to personalized therapy of obesity. PBMC were collected from obese men before and after an 8-week low-calorie diet (LCD) to lose weight. Changes in gene expression before and after the LCD were initially screened using a DNA-microarray platform and validated by qRT-PCR. Global gene expression analysis identified 385 differentially expressed transcripts after the LCD. Further analyses showed a decrease in some specific oxidative stress and inflammation genes. Interestingly, expression of these genes was directly related to body weight, while a lower IL8 gene expression was associated with higher fat mass decrease. Collectively, these observations suggest that PBMCs are a suitable RNA source and model system to perform nutrigenomics studies related to obesity and development of personalized dietary treatments. IL8 gene expression warrant further research as a putative novel biomarker of changes in body fat percentage in response to an LCD.
Authors	Ana B Crujeiras, Dolores Parra, Fermín I Milagro, Estibaliz Goyenechea, Eider Larrarte, Javier Margareto, J Alfredo Martínez
Journal	Omics : a journal of integrative biology (OMICS) Vol. 12 Issue 4 Pg. 251-61 (Dec 2008) ISSN: 1557-8100 [Electronic] United States
PMID	18687040 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	CXCL8 protein, human Genetic Markers Inflammation Mediators Interleukin-8
Topics	Adult Caloric Restriction Gene Expression Profiling Genetic Markers Humans Inflammation Mediators (metabolism) Interleukin-8 (genetics, metabolism) Leukocytes, Mononuclear (immunology) Male Middle Aged Nutrigenomics Obesity (genetics, metabolism) Oligonucleotide Array Sequence Analysis Oxidative Stress Weight Loss (genetics)

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