Effect of xanthohumol on melanogenesis in B16 melanoma cells.

Abstract

Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited alpha-melanocyte stimulating hormone- or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia-associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.

• Authors: Jeung-Hyun Koo, Hyoung Tae Kim, Ha-Yong Yoon, Kang-Beom Kwon, Il-Whan Choi, Sung Hoo Jung, Han-Uk Kim, Byung-Hyun Park, Jin-Woo Park
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 40 Issue 3 Pg. 313-9 (Jun 30 2008) ISSN: 1226-3613 [Print] United States
• PMID: 18587269 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Melanins
• Membrane Glycoproteins
• Microphthalmia-Associated Transcription Factor
• Mitf protein, mouse
• Propiophenones
• Colforsin
• alpha-MSH
• Oxidoreductases
• Tyrp1 protein, mouse
• Monophenol Monooxygenase
• Intramolecular Oxidoreductases
• dopachrome isomerase
• xanthohumol
• 1-Methyl-3-isobutylxanthine

Topics

• 1-Methyl-3-isobutylxanthine (pharmacology)
• Animals
• Cell Line
• Cell Survival (drug effects)
• Colforsin (pharmacology)
• Dose-Response Relationship, Drug
• Down-Regulation
• Drug Antagonism
• Flavonoids
• Humulus
• Intramolecular Oxidoreductases (antagonists & inhibitors, biosynthesis)
• Melanins (antagonists & inhibitors, biosynthesis)
• Melanocytes (drug effects, metabolism)
• Melanoma, Experimental
• Membrane Glycoproteins (antagonists & inhibitors, biosynthesis)
• Mice
• Microphthalmia-Associated Transcription Factor (antagonists & inhibitors, biosynthesis)
• Monophenol Monooxygenase (antagonists & inhibitors, biosynthesis, genetics)
• Oxidoreductases (antagonists & inhibitors, biosynthesis)
• Propiophenones (pharmacology)
• Signal Transduction (drug effects)
• alpha-MSH (metabolism)