Abstract | BACKGROUND:
Photodynamic therapy ( PDT) involves the administration of a tumor-localizing photosensitizing drug, which is activated by light of specific wavelength in the presence of molecular oxygen thus generating reactive oxygen species that is toxic to the tumor cells. PDT selectively destroys photosensitized tissue leading to various cellular and molecular responses. The present study was designed to examine the angiogenic responses at short (0.5 h) and long (6 h) drug light interval (DLI) hypericin- PDT (HY- PDT) treatment at 24 h and 30 days post treatment in a human bladder carcinoma xenograft model. As short DLI targets tumor vasculature and longer DLI induces greater cellular damage, we hypothesized a differential effect of these treatments on the expression of angiogenic factors. RESULTS: CONCLUSION: In conclusion, long DLI HY- PDT induces upregulation of angiogenic proteins. Differential expression of genes involved in the angiogenesis pathway was observed in the various groups treated with HY- PDT.
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Authors | Ramaswamy Bhuvaneswari, Yik Y Gan, Sasidharan S Lucky, William W L Chin, Seyed M Ali, Khee C Soo, Malini Olivo |
Journal | Molecular cancer
(Mol Cancer)
Vol. 7
Pg. 56
(Jun 13 2008)
ISSN: 1476-4598 [Electronic] England |
PMID | 18549507
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenic Proteins
- Anthracenes
- Photosensitizing Agents
- Perylene
- hypericin
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Topics |
- Angiogenic Proteins
(genetics, metabolism)
- Animals
- Anthracenes
- Blotting, Western
- Cell Line, Tumor
- Endoscopy
(methods)
- Fluorescence
- Gene Expression Profiling
- Humans
- Immunohistochemistry
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Microscopy, Confocal
- Neovascularization, Pathologic
(drug therapy, genetics, metabolism, pathology)
- Perylene
(analogs & derivatives, pharmacology)
- Photochemotherapy
- Photosensitizing Agents
(pharmacology)
- Polymerase Chain Reaction
- Protein Array Analysis
- Time Factors
- Urinary Bladder Neoplasms
(blood supply, drug therapy, genetics, metabolism, pathology)
- Xenograft Model Antitumor Assays
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