Anti-inflammatory effect of SQC-beta-CD on lipopolysaccharide-induced acute lung injury.

Abstract

AIM OF THE STUDY:

Shuang-Qing-Cao (SQC) is a folk Chinese medicinal formula. The therapeutic effects of inclusion complexation of SQC extract in beta-cyclodextrin (SQC-beta-CD) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) were studied in mice.

MATERIALS AND METHODS:

Two protocols were designed for administration of SQC-beta-CD (10 and 20 mg/kg body weight) or DEX (2 mg/kg). According to Protocol A we intraperitoneally injected diluent (saline with 0.5% Tween 80), SQC-beta-CD or DEX respectively into mice 30 min and 3h after LPS challenge. Alternatively, in Protocol B we administered diluent, SQC-beta-CD or DEX 3h before and 30 min after LPS challenge.

RESULTS:

The histological results showed that SQC-beta-CD (20 mg/kg) protected mice from LPS-induced ALI such as oedema, haemorrhage, blood vessel and alveolar structural damage. Furthermore, SQC-beta-CD inhibited LPS-increased pulmonary MPO activity and migration of polymorphonuclear neutrophils (PMNs) into bronchoalveolar lavage fluid (BALF). Immunohistochemical experiment demonstrated that SQC-beta-CD decreased inducible nitric oxide synthase (iNOS) expression in lung 24h after LPS administration. Consequently, SQC-beta-CD prevented LPS-induced nitric oxide (NO) release in BALF.

CONCLUSIONS:

The results indicated that SQC-beta-CD is greatly effective in inhibiting ALI. The present study indicated that SQC-beta-CD acted as a potential therapeutic reagent for treating ALI.

• Authors: Qin Feng, Yong Ren, Yu Wang, Hsiaoyen Ma, Jun Xu, Chenrong Zhou, Zhimin Yin, Lan Luo
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 118 Issue 1 Pg. 51-8 (Jun 19 2008) ISSN: 0378-8741 [Print] Ireland
• PMID: 18495394 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Drugs, Chinese Herbal
• Lipopolysaccharides
• Plant Extracts
• beta-Cyclodextrins
• Nitric Oxide
• Dexamethasone
• Nitric Oxide Synthase Type II
• betadex

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Dexamethasone (pharmacology)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (administration & dosage, chemistry, pharmacology)
• Lipopolysaccharides
• Male
• Mice
• Mice, Inbred ICR
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (drug effects, metabolism)
• Plant Extracts (administration & dosage, chemistry, pharmacology)
• Respiratory Distress Syndrome (chemically induced, drug therapy, physiopathology)
• Time Factors
• beta-Cyclodextrins (chemistry)