Abstract | BACKGROUND: The therapeutic potential of bone marrow (BM)-derived cells in ulcers is not known. This study aimed to clarify (1) cell types that are derived from the BM which infiltrate ulcers; (2) whether BM-derived cells or gastric myofibroblasts can be used for cell transplantation to treat ulcers; and (3) the phenotypes of such transplantable cells. METHODS: RESULTS: (1) GFP-positive cells with interstitial phenotypes were observed at the ulcerated area. (2) Ulcer healing was significantly promoted by the injection of BM-derived cells compared to controls on day 7, but not on day 3. The BM-derived cells were observed in the tissue surrounding the ulcer. However, myofibroblasts were not found. (3) The BM-derived cells expressed hepatocyte growth factor, transforming growth factor-beta(1), and other stromal factors before transplantation, and had mesenchymal phenotypes after transplantation. CONCLUSIONS: BM-derived cells are involved in the ulcer healing. BM-derived cells, but not myofibroblasts, are locally implantable to ulcers. Thus, BM-derived cells can be transplanted to accelerate ulcer healing.
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Authors | Tsutomu Nishida, Shingo Tsuji, Masahiko Tsujii, Shuji Ishii, Toshiyuki Yoshio, Shinichiro Shinzaki, Satoshi Egawa, Takanobu Irie, Yoshimi Kakiuchi, Masakazu Yasumaru, Hideki Iijima, Shusaku Tsutsui, Sunao Kawano, Norio Hayashi |
Journal | Journal of gastroenterology
(J Gastroenterol)
Vol. 43
Issue 2
Pg. 124-35
( 2008)
ISSN: 0944-1174 [Print] Japan |
PMID | 18306986
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Acetic Acid
(adverse effects)
- Animals
- Bone Marrow Transplantation
- Cell Transdifferentiation
- Cells, Cultured
- Female
- Male
- Mice
- Mice, Transgenic
- Phenotype
- Stomach Ulcer
(chemically induced, physiopathology, surgery)
- Wound Healing
(physiology)
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