TCDD exposure exacerbates atopic dermatitis-related inflammation in NC/Nga mice.

Abstract

Our previous study showed that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure of NC/Nga mice, a mouse model of atopic dermatitis, induces no dermal changes. In the present study, to investigate whether TCDD exacerbates atopic dermatitis-like skin lesions elicited in NC/Nga mice, NC/Nga mice were applied with picryl chloride (PC), and then were exposed to a single oral dose of 0 (control), 5, and 20 microg TCDD/kg. Two weeks later, spleens, blood, and skin specimens were collected. TCDD exposure increased the production of Th1-type cytokine IFN-gamma, but not Th2-type cytokine IL-4, from spleen cells stimulated with a mitogen. The plasma total IgE antibody levels of the TCDD-exposed mice remained at control levels. On the other hand, TCDD exposure markedly increased the mast cell infiltration and degranulation in PC-sensitized NC/Nga mice histologically, as compared with control mice. These results suggest that TCDD exposure exacerbates atopic dermatitis-related inflammation with no increase of IgE antibody production and that TCDD may be one of the environmental pollutants that induce exacerbations of atopic diseases.

• Authors: Tomohiro Ito, Kaoru Inouye, Keiko Nohara, Chiharu Tohyama, Hidekazu Fujimaki
• Journal: Toxicology letters (Toxicol Lett) Vol. 177 Issue 1 Pg. 31-7 (Feb 28 2008) ISSN: 0378-4274 [Print] Netherlands
• PMID: 18272299 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Environmental Pollutants
• Polychlorinated Dibenzodioxins
• Immunoglobulin E
• Picryl Chloride

Topics

• Animals
• Cells, Cultured
• Cytokines (metabolism)
• Dermatitis, Atopic (chemically induced, metabolism, pathology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Synergism
• Drug Therapy, Combination
• Ear Auricle (drug effects, pathology)
• Environmental Pollutants (toxicity)
• Immunoglobulin E (blood)
• Mice
• Mice, Inbred Strains
• Organ Size (drug effects)
• Picryl Chloride (toxicity)
• Polychlorinated Dibenzodioxins (toxicity)
• Skin (drug effects, metabolism, pathology)
• Spleen (drug effects, pathology)