Abstract | OBJECTIVE: METHODS: [(18)F]-5-FU was made with >95% radiochemical purity in our laboratory. BALB/cAJcl-nu/nu mice were subcutaneously inoculated with colon carcinoma cell line, colon 26, into the left side of the back and turpentine oil into the right side of the back to cause chemical inflammation. We examined the biodistribution of [(18)F]-5-FU in control mice and tumor- inflammation mice. We also examined the biodistribution of [(18)F]-FDG as a baseline study. Approximately 1 MBq of either [(18)F]-5-FU or [(18)F]-FDG was injected into the tail vein of each mouse. The biodistribution study was performed at 1 and 2 h after injection. The radioactivity of each organ was measured by a gamma counter. RESULTS: [(18)F]-5-FU uptakes in the liver and the kidney were especially high. Tumor-to-blood ratios were significantly higher at 2 h than at 1 h (3.69 +/- 0.40 vs. 1.81 +/- 0.37, P < 0.001). Tumor-to- inflammation ratios at 2 h following injection were significantly higher than those at 1 h (1.94 +/- 0.44 vs. 1.26 +/- 0.20, P < 0.001). At 2 h after radiotracer injection, the tumor-to- inflammation ratio of [(18)F]-5-FU was significantly higher than that of [(18)F]-FDG (1.94 +/- 0.44 vs. 1.03 +/- 0.23, P = 0.001). CONCLUSIONS: Our data suggest that [(18)F]-5-FU has a diagnostic potential as a positron emission tomography ligand for differentiating malignant tumors from inflammatory lesions.
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Authors | Sadatoshi Sugae, Akiko Suzuki, Nobukazu Takahashi, Ryogo Minamimoto, Chao Cheng, Chumpol Theeraladanon, Itaru Endo, Shinji Togo, Tomio Inoue, Hiroshi Shimada |
Journal | Annals of nuclear medicine
(Ann Nucl Med)
Vol. 22
Issue 1
Pg. 65-72
(Jan 2008)
ISSN: 0914-7187 [Print] Japan |
PMID | 18250989
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorine Radioisotopes
- Radiopharmaceuticals
- Fluorouracil
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Topics |
- Animals
- Colonic Neoplasms
(diagnostic imaging, metabolism)
- Diagnosis, Differential
- Female
- Fluorine Radioisotopes
(pharmacokinetics)
- Fluorouracil
(pharmacokinetics)
- Inflammation
(diagnostic imaging, metabolism)
- Metabolic Clearance Rate
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Organ Specificity
- Positron-Emission Tomography
(methods)
- Radiopharmaceuticals
(pharmacokinetics)
- Reproducibility of Results
- Sensitivity and Specificity
- Tissue Distribution
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