Evidence for concerted action of FAT/CD36 and FABPpm to increase fatty acid transport across the plasma membrane.

Abstract

There is substantial molecular, biochemical and physiologic evidence that long-chain fatty acid transport involves a protein-mediated process. A number of fatty acid transport proteins have been identified, and for unknown reasons, some of these are coexpressed in the same tissues. In muscle and heart FAT/CD36 and FABPpm appear to be key transporters. Both proteins are regulated acutely (within minutes) and chronically (hours to days) by selected physiologic stimuli (insulin, AMP kinase activation). Acute regulation involves the translocation of FAT/CD36 by insulin, muscle contraction and AMP kinase activation, while FABPpm is induced to translocate by muscle contraction and AMP kinase activation, but not by insulin. Protein expression ofFAT/CD36 and FABPpm is regulated by prolonged AMP kinase activation (heart) or increased muscle contraction. Prolonged insulin exposure increases the expression of FAT/CD36 but not FABPpm. Trafficking of fatty acid transporters between an intracellular compartment(s) and the plasma membrane is altered in insulin-resistant skeletal muscle, as some FAT/CD36 is permanently relocated to plasma membrane, thereby contributing to insulin resistance due to the increased influx of fatty acids into muscle cells. Studies in FAT/CD36 null mice have revealed that this transporter is key to regulating the increase in the rate of fatty acid metabolism in heart and skeletal muscle. It appears based on a number of experiments that FAT/CD36 and FABPpm may collaborate to increase the rates of fatty acid transport, as these proteins co-immunoprecipitate.

• Authors: Adrian Chabowski, Jan Górski, Joost J F P Luiken, Jan F C Glatz, Arend Bonen
• Journal: Prostaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) 2007 Nov-Dec Vol. 77 Issue 5-6 Pg. 345-53 ISSN: 0952-3278 [Print] Scotland
• PMID: 18240411 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)

Chemical References

• CD36 Antigens
• Fatty Acid-Binding Proteins
• Fatty Acids

Topics

• Animals
• Biological Transport (physiology)
• CD36 Antigens (metabolism, physiology)
• Cell Membrane (metabolism)
• Fatty Acid-Binding Proteins (metabolism, physiology)
• Fatty Acids (metabolism)
• Humans