Anti-inflammatory effect of alpha, beta-Amyrin, a pentacyclic triterpene from Protium heptaphyllum in rat model of acute periodontitis.

Abstract

This study was aimed to evaluate the anti-inflammatory potential of triterpene alpha, beta-amyrin in rats on acute phase periodontitis. Periodontitis was induced by ligature placement around the maxillary right second molar tooth. Rats (n = 8/group) were pretreated with alpha, beta-amyrin (5 and 10 mg/kg, p. o.), two hours before the induction of periodontal inflammation. Sham-operated and positive controls (lumiracoxib and dexamethasone) were included. Six hours later, plasma levels of TNF-alpha were analysed. Rats were sacrificed at 24 h, and the gingival tissue analysed for myeloperoxidase (MPO) and thiobarbituric acid-reactive substances (TBARS), as measures of neutrophil influx and lipid-peroxidation, respectively alpha, beta-Amyrin as well as dexamethasone significantly inhibited the periodontitis-associated increases of TNF-alpha, and the gingival MPO and TBARS. alpha, beta-Amyrin effect was more prominent at 5 mg/kg. Lumiracoxib manifested varied influence on the studied parameters. These results provide evidence to show that alpha, beta-Amyrin retards acute inflammation in rat model of periodontitis and warrant further study on its efficacy to prevent chronic periodontitis-associated bone loss.

• Authors: S A Holanda Pinto, L M S Pinto, G M A Cunha, M H Chaves, F A Santos, V S Rao
• Journal: Inflammopharmacology (Inflammopharmacology) Vol. 16 Issue 1 Pg. 48-52 (Feb 2008) ISSN: 0925-4692 [Print] Switzerland
• PMID: 18046512 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Pentacyclic Triterpenes
• Thiobarbituric Acid Reactive Substances
• Tumor Necrosis Factor-alpha
• Diclofenac
• Oleanolic Acid
• Dexamethasone
• Peroxidase
• beta-amyrin
• lumiracoxib

Topics

• Acute Disease
• Administration, Oral
• Animals
• Anti-Inflammatory Agents (administration & dosage, chemistry, pharmacology)
• Burseraceae (chemistry)
• Dexamethasone (administration & dosage, pharmacology)
• Diclofenac (administration & dosage, analogs & derivatives, pharmacology)
• Disease Models, Animal
• Gingiva (drug effects, metabolism, pathology)
• Gingivitis (metabolism, pathology, prevention & control)
• Isomerism
• Male
• Molecular Structure
• Neutrophil Infiltration (drug effects)
• Oleanolic Acid (administration & dosage, analogs & derivatives, chemistry, pharmacology)
• Oxidative Stress (drug effects)
• Pentacyclic Triterpenes (administration & dosage, chemistry, pharmacology)
• Periodontitis (metabolism, pathology, prevention & control)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar
• Thiobarbituric Acid Reactive Substances (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)