A novel homospermidine conjugate inhibits growth and induces apoptosis in human hepatoma cells.

Abstract

AIM:

To elucidate the mechanism responsible for the antiproliferative effects of a novel homospermidine conjugate, anthracenylmethyl homospermidine (ANTMHspd), in the human hepatoma BEL-7402 cell line.

METHODS:

The viability of the cells was assessed by MTT assay and the trypan blue dye exclusion method. Morphological changes were observed by fluorescence microscopy with Hoechst 33258 staining. Cell cycle distribution, apoptosis, and mitochondrial membrane potential were measured by flow cytometry. Protein expression was detected by Western blot analysis.

RESULTS:

ANTMHspd strongly decreased BEL-7402 cell proliferation in a dose- and time-dependent manner. Hoechst 33258 staining and the flow cytometry assay showed that ANTMHspd induced cell apoptosis and cell cycle perturbation. Furthermore, ANTMHspd could induce mitochondrial membrane potential loss and cytochrome c release and enhance cleaved caspase-3, cleaved caspase-9, and Bax protein expression without caspase-8 activation. ANTMHspd could also decrease the expression of Bcl-2 and cytochrome c in mitochondria. In addition, the specific inhibitors of caspase-9 and caspase-3 almost abolished the ANTMHspd-induced caspase-9 and caspase-3 activation, respectively.

CONCLUSION:

ANTMHspd could induce BEL-7402 cell apoptosis via the mitochondrial/caspase-dependent pathway and the Bcl-2 family was involved in the control of apoptosis.

• Authors: Song-qiang Xie, Ying-liang Wu, Peng-fei Cheng, Min-wei Wang, Guang-chao Liu, Yuan-fang Ma, Jin Zhao, Chao-jie Wang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 28 Issue 11 Pg. 1827-34 (Nov 2007) ISSN: 1671-4083 [Print] United States
• PMID: 17959035 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthracenes
• Proto-Oncogene Proteins c-bcl-2
• anthracenylmethyl homospermidine
• bcl-2-Associated X Protein
• Cytochromes c
• Caspases
• Spermidine

Topics

• Anthracenes (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (drug therapy, pathology)
• Caspases (metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cytochromes c (metabolism)
• Dose-Response Relationship, Drug
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Spermidine (analogs & derivatives, pharmacology)
• Time Factors
• bcl-2-Associated X Protein (metabolism)