Abstract | BACKGROUND AND OBJECTIVE: MATERIALS AND METHODS: A coculture of human pulmonary microvascular endothelial cells (HMVEC) and monocytes in the presence of serum containing anti- HLA DR alloantibodies previously associated with cases of TRALI was used as an in vitro TRALI model. The release of leukotriene B(4) (LTB(4)) and tumour necrosis factor-alpha ( TNF-alpha), the apoptosis of HMVECs were measured. RESULTS: The release of LTB(4) and TNF-alpha and apoptosis of HMVECs were observed in the model. The addition of psHLA DR markedly reduced the release of LTB(4) and TNF-alpha and inhibited apoptosis of HMVECs. CONCLUSION:
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Authors | M Nishimura, S Hashimoto, M Satake, H Okazaki, K Tadokoro |
Journal | Vox sanguinis
(Vox Sang)
Vol. 93
Issue 1
Pg. 78-82
(Jul 2007)
ISSN: 0042-9007 [Print] England |
PMID | 17547569
(Publication Type: Journal Article)
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Chemical References |
- HLA-DR Antigens
- Isoantibodies
- Tumor Necrosis Factor-alpha
- Leukotriene B4
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Topics |
- Antibody Formation
(drug effects, immunology)
- Apoptosis
(drug effects, immunology)
- Cells, Cultured
- Endothelial Cells
(immunology, pathology)
- HLA-DR Antigens
(immunology, isolation & purification, pharmacology, therapeutic use)
- Humans
- Isoantibodies
(immunology)
- Leukotriene B4
(immunology)
- Lung
(blood supply, immunology, pathology)
- Models, Biological
- Monocytes
(immunology, pathology)
- Neutrophils
(immunology, pathology)
- Respiratory Distress Syndrome
(drug therapy, etiology, immunology, pathology)
- Tumor Necrosis Factor-alpha
(immunology)
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