Abstract | OBJECTIVE: METHODS: RESULTS: The ratio of CD133 positive cells with stem cell property was 0.5% in U87 cells. Activation of CXCR4 on glioma stem cells induced calcium mobilization and increased VEGF and IL-8 protein secretion. CD133 positive cells secreted more VEGF and IL-8 than their negative counterparts in vitro. Tumors derived from CD133 positive cells grew more rapidly and expressed elevated level of VEGF than their negative counterparts. CONCLUSIONS: There are a small fraction of glioma stem cells in human glioblastoma cell line U87. Expressing functional CXCR4 and secreting more pro-angiogenic factors may be involved in tumor angiogenesis mediated by glioma stem cells.
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Authors | Yi-fang Ping, Xiao-hong Yao, Xiu-wu Bian, Jian-hong Chen, Rong Zhang, Liang Yi, Zhi-hua Zhou |
Journal | Zhonghua bing li xue za zhi = Chinese journal of pathology
(Zhonghua Bing Li Xue Za Zhi)
Vol. 36
Issue 3
Pg. 179-83
(Mar 2007)
ISSN: 0529-5807 [Print] China |
PMID | 17535685
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AC133 Antigen
- Antigens, CD
- Glial Fibrillary Acidic Protein
- Glycoproteins
- Interleukin-8
- Intermediate Filament Proteins
- NES protein, human
- Nerve Tissue Proteins
- Nes protein, mouse
- Nestin
- PROM1 protein, human
- Peptides
- Prom1 protein, mouse
- Receptors, CXCR4
- Vascular Endothelial Growth Factor A
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Topics |
- AC133 Antigen
- Animals
- Antigens, CD
(analysis)
- Brain Neoplasms
(blood supply, metabolism, pathology)
- Cell Line, Tumor
- Female
- Gene Expression Regulation, Neoplastic
- Glial Fibrillary Acidic Protein
(metabolism)
- Glioblastoma
(blood supply, metabolism, pathology)
- Glycoproteins
(analysis)
- Humans
- Interleukin-8
(metabolism)
- Intermediate Filament Proteins
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- Neoplastic Stem Cells
(metabolism, transplantation)
- Neovascularization, Pathologic
- Nerve Tissue Proteins
(metabolism)
- Nestin
- Peptides
(analysis)
- Receptors, CXCR4
(genetics, metabolism, physiology)
- Vascular Endothelial Growth Factor A
(metabolism)
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