Protective effects of trimetazidine on testicular ischemia-reperfusion injury in rats.

Abstract

INTRODUCTION:

We tried to prove the effectiveness of trimetazidine (TMZ) on testicular torsion-detorsion injury.

MATERIALS AND METHODS:

15 male rats were equally divided into three groups: group 1 was the sham-operated control group; group 2 had 2 h of unilateral testicular ischemia followed by 3 days of reperfusion, and group 3 had 2 h of unilateral testicular ischemia followed by 3 days of oral TMZ treatment (5 mg/kg, bid) during reperfusion. In the removed testicles, tissue reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), malondialdehyde (MDA) levels and pathological modified Johnson scores (MJS) were evaluated. Mann-Whitney U test was used for statistical evaluations.

RESULTS:

In group 2, on the ipsilateral side, GSH were significantly lower and MDA were higher than in groups 1 and 3, though GSH and MDA were not statistically different between groups 1 and 3. On the other hand, GPx in the control testicles of group 3 was significantly lower compared to those in the counterparts of both groups 1 and 2. Among three groups, GR determined in both testicles were not statistically different. On the ipsilateral side, MJS in group 3 were lower than in the sham group, but significantly higher than in group 2.

CONCLUSIONS:

According to this study, TMZ has an antioxidant effect on testicular torsion-detorsion injury, though the protective effect of TMZ seems to decrease in control testicles. Consequently it has been considered that TMZ can be only used in torsion patients with a healthy contralateral testicle after further studies have been conducted.

• Authors: Dogan Unal, Omer F Karatas, Murat Savas, Ercan Yeni, Bulent S Keser, Ayhan Verit, Ozcan Erel, Muharrem Bitiren
• Journal: Urologia internationalis (Urol Int) Vol. 78 Issue 4 Pg. 356-62 ( 2007) ISSN: 0042-1138 [Print] Switzerland
• PMID: 17495496 (Publication Type: Clinical Trial, Comparative Study, Journal Article)

Chemical References

• Vasodilator Agents
• Trimetazidine

Topics

• Animals
• Male
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy)
• Testis (drug effects, pathology)
• Treatment Outcome
• Trimetazidine (pharmacology)
• Vasodilator Agents (pharmacology)