Pro12Ala polymorphism of the peroxisome proliferator-activated receptor-gamma2 (PPARgamma-2) gene is associated with greater insulin sensitivity and decreased risk of type 2 diabetes in an Iranian population.

Abstract

BACKGROUND:

The Pro12Ala polymorphism of the peroxisome proliferator-activated receptor-gamma2 (PPARgamma-2) gene has been variably associated with insulin resistance, obesity and type 2 diabetes in several populations. However, this association has not been studied in Iranian subjects and we hypothesized that this variation might be associated with insulin resistance, type 2 diabetes and related metabolic traits in this population.

METHODS:

The Pro12Ala genotypes were determined by PCR-restriction fragment length polymorphism in 696 unrelated subjects including 412 non-diabetic controls and 284 type 2 diabetic patients.

RESULTS:

The frequency of the Ala allele was 9.4% and 5.9% in controls and type 2 diabetic subjects, respectively [adjusted odds ratio (OR) 0.457, p=0.005]. The Ala allele did not show a significant effect on anthropometric and biochemical parameters in the type 2 diabetic group, whereas in non-diabetic subjects, carriers of the Ala allele had significantly lower fasting insulin (p=0.007) and homeostasis model assessment of insulin resistance (HOMA-IR) (p=0.009) levels compared to Pro/Pro subjects. Multivariate logistic regression analysis showed that Pro12Ala polymorphism was an independent determinant of type 2 diabetes in this population.

CONCLUSIONS:

Our results for a sample of Iranian type 2 diabetes cases and controls provide evidence that the Pro/Ala genotype of the PPARgamma-2 gene is associated with insulin sensitivity and may also have protective role against type 2 diabetes.

• Authors: Reza Meshkani, Mohammad Taghikhani, Bagher Larijani, Yadollah Bahrami, Shohreh Khatami, Ehteram Khoshbin, Amir Ghaemi, Sedigheh Sadeghi, Fatemeh Mirkhani, Azam Molapour, Khosrow Adeli
• Journal: Clinical chemistry and laboratory medicine (Clin Chem Lab Med) Vol. 45 Issue 4 Pg. 477-82 ( 2007) ISSN: 1434-6621 [Print] Germany
• PMID: 17439324 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• PPAR gamma
• Proline
• Alanine

Topics

• Alanine (genetics)
• Base Sequence
• DNA Primers
• Diabetes Mellitus, Type 2 (genetics)
• Genetic Predisposition to Disease
• Humans
• Insulin Resistance (genetics)
• Iran
• PPAR gamma (genetics)
• Polymorphism, Genetic
• Proline (genetics)