Abstract | UNLABELLED:
Sepsis alone and burn complicated by sepsis produce significant cardiac dysfunction. Since calcium handling by the cardiomyocyte is essential for cardiac function, one mechanism for cardiac abnormalities may be calcium dyshomeostasis. We hypothesized that sepsis and burn plus sepsis alter cardiac calcium transporter expression. Sprague-Dawley rats were divided into: (1) control, (2) sepsis (intratracheal S. Pneumoniae, 4x10(6) CFU), and (3) burn (40% TBSA) plus sepsis. Myocyte [Ca(2+)](i) and [Na(+)](i) were quantified with Fura-2 AM and SBFI, respectively. Western blot analysis of rat hearts used antibodies against the sarcoplasmic reticular Ca(2+) ATPase (SERCA), the L-type calcium channel, the Na(+)/Ca(2+) exchanger or the Na(+)/K(+) ATPase. RESULTS:
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Authors | Cherry Ballard-Croft, David L Maass, Patricia J Sikes, Jureta W Horton |
Journal | Burns : journal of the International Society for Burn Injuries
(Burns)
Vol. 33
Issue 1
Pg. 72-80
(Feb 2007)
ISSN: 0305-4179 [Print] Netherlands |
PMID | 17137718
(Publication Type: Journal Article)
|
Chemical References |
- Sodium
- Calcium-Transporting ATPases
- Sodium-Potassium-Exchanging ATPase
|
Topics |
- Animals
- Blotting, Western
- Burns
(metabolism)
- Calcium-Transporting ATPases
(metabolism)
- Myocytes, Cardiac
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Sepsis
(metabolism)
- Sodium
(metabolism)
- Sodium-Potassium-Exchanging ATPase
(metabolism)
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