Peroxisome proliferator-activated receptor gamma is highly expressed in pancreatic cancer and is associated with shorter overall survival times.

Abstract

PURPOSE:

Peroxisome proliferator-activated receptor gamma (PPARgamma) is a ligand-activated transcription factor that has been implicated in carcinogenesis and progression of various solid tumors, including pancreatic carcinoma. We aimed to clarify the expression patterns of PPARgamma in pancreatic ductal carcinomas and to correlate these to clinicopathologic variables, including patient survival.

EXPERIMENTAL DESIGN:

Array-based expression profiling of 19 microdissected carcinomas and 14 normal ductal epithelia was conducted. Additionally, Western blots of pancreatic cancer cell lines and paraffinized tissue of 129 pancreatic carcinomas were immunostained for PPARgamma. For statistical analysis, Fisher's exact test, chi2 test for trends, correlation analysis, Kaplan-Meier analysis, and Cox's regression were applied.

RESULTS:

Expression profiles showed a strong overexpression of PPARgamma mRNA (change fold, 6.9; P=0.04). Immunohistochemically, PPARgamma expression was seen in 71.3% of pancreatic cancer cases. PPARgamma expression correlated positively to higher pT stages and higher tumor grade. Survival analysis showed a significant prognostic value for PPARgamma, which was found to be independent in the clinically important subgroup of node-negative tumors.

CONCLUSIONS:

PPARgamma is commonly up-regulated in pancreatic ductal adenocarcinoma and might be a prognostic marker in this disease. Both findings corroborate the importance of PPARgamma in tumor progression of pancreatic cancer.

• Authors: Glen Kristiansen, Juliane Jacob, Ann-Christin Buckendahl, Robert Grützmann, Ingo Alldinger, Bence Sipos, Günter Klöppel, Marcus Bahra, Jan M Langrehr, Peter Neuhaus, Manfred Dietel, Christian Pilarsky
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 12 Issue 21 Pg. 6444-51 (Nov 01 2006) ISSN: 1078-0432 [Print] United States
• PMID: 17085658 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• PPAR gamma
• RNA, Messenger

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Carcinoma, Pancreatic Ductal (metabolism, mortality)
• Female
• Gene Expression
• Gene Expression Profiling
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• PPAR gamma (genetics, metabolism)
• Pancreatic Neoplasms (metabolism, mortality)
• RNA, Messenger (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• Survival Analysis