Abstract | BACKGROUND: METHODS: Three poly- vinyl chloride containers of each dialysis fluid were stored at 38 degrees C for 4 days. Samples were taken after the bags were mixed and allowed to stand for 2 minutes. Two 500-microL samples were collected from each bag at hours 0, 12, 24, 36, 48, 60, 72, 84, and 96. Samples were then analyzed by high-performance liquid chromatography (HPLC) in our laboratory. In order to establish the stability-indicating nature of the method, drugs 1.0 mg/mL, vehicles, and their mixtures were subjected to a forced degradation. This is done by acid (2.0 mol/L HCl) and base (2.0 mol/L NaOH) hydrolysis, oxidation (H(2)O(2) 0.3%), and heat at 80 degrees C. Samples were analyzed every 30 minutes until approximately 25% of the drug's peak disappeared. The drug was considered stable if its concentration exceeded 90% of the original. RESULTS: CONCLUSION:
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Authors | Renee F Robinson, Richard S Morosco, Charles V Smith, John D Mahan |
Journal | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis
(Perit Dial Int)
2006 Sep-Oct
Vol. 26
Issue 5
Pg. 593-7
ISSN: 0896-8608 [Print] United States |
PMID | 16973516
(Publication Type: Journal Article)
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Chemical References |
- Dialysis Solutions
- Heparin
- Cefazolin
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Topics |
- Cefazolin
(analysis)
- Dialysis Solutions
(chemistry)
- Drug Stability
- Heparin
(pharmacology)
- Humans
- In Vitro Techniques
- Temperature
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