Abstract | BACKGROUND:
Glycyrrhizin, an agent that can bind to selectins and inhibit their ability to bind neutrophils, was found to be effective in preventing tissue edema caused by ischemia-reperfusion in a rabbit model. METHODS: Complete ischemia was produced by applying a tight Esmarch tourniquet to the hind limbs of 24 Japanese white rabbits. Immediately before and 1 h after release of the tourniquet, 12 animals were given glycyrrhizin intravenously; 12 controls received saline. RESULTS: The mean relative increase in the circumference of the shins before and after ischemia-reperfusion with or without glycyrrhizin treatment was 4.6% +/- 2.4% and 9.6% +/- 4.2%, respectively, indicating that tissue edema caused by the ischemia-reperfusion was significantly attenuated by glycyrrhizin. Histological studies of cross sections of the anterior tibial muscle 24 h after reperfusion showed a significant reduction in the incidence of necrotic muscle fibers in the glycyrrhizin-treated animals compared with the controls that did not receive glycyrrhizin. The mRNA levels of P- and E-selectin 24 h after reperfusion were significantly higher in the ischemic anterior tibial muscle than in the nonischemic normal muscle. After 24 h of reperfusion, the mean activity of myeloperoxidase, a neutrophil-specific enzyme, in the anterior tibial muscles of the group given glycyrrhizin (0.0022 +/- 0.0013 absorbance units) was lower than that of the untreated group (0.027 +/- 0.026 absorbance units). CONCLUSIONS:
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Authors | Nobuaki Nakata, Kunio Takaoka |
Journal | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association
(J Orthop Sci)
Vol. 11
Issue 4
Pg. 375-9
(Jul 2006)
ISSN: 0949-2658 [Print] Japan |
PMID | 16897202
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- RNA, Messenger
- Selectins
- Glycyrrhizic Acid
- Peroxidase
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(therapeutic use)
- Capillaries
(metabolism)
- Edema
(prevention & control)
- Endothelium, Vascular
(metabolism)
- Glycyrrhizic Acid
(therapeutic use)
- Hindlimb
- Peroxidase
(metabolism)
- RNA, Messenger
(metabolism)
- Rabbits
- Reperfusion Injury
(pathology, prevention & control)
- Reverse Transcriptase Polymerase Chain Reaction
- Selectins
(metabolism)
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