Overexpression of SOD1 in transgenic rats attenuates nuclear translocation of endonuclease G and apoptosis after spinal cord injury.

Abstract

Spinal motor neurons are selectively vulnerable after spinal cord injury (SCI). Recent studies suggest they undergo apoptosis after caspase activation through a mitochondria-dependent apoptosis pathway, and that oxidative stress after SCI is likely to play a role. However, other signaling pathways of apoptosis that involve mitochondria have not been thoroughly studied after SCI. Apoptosis-inducing factor (AIF) and endonuclease G (EndoG) are mitochondrial apoptogenic proteins that are capable of inducing neuronal apoptosis when translocated from mitochondria to nuclei through a caspase-independent pathway. In this study, we examined translocation of these proteins and apoptotic cell death of motor neurons. The role of oxidative stress was also studied using transgenic (Tg) rats that overexpress the intrinsic antioxidant copper/zinc-superoxide dismutase (SOD1). Western blots and an activity assay demonstrated that a greater amount of SOD1 and higher activity of SOD presented in mitochondria of Tg rats compared with wild-type (Wt) rats. Immunohistochemistry and Western blots showed translocation of EndoG and AIF from mitochondria to nuclei in motor neurons 1 day after SCI in both groups of rats. However, there was significantly less translocation of EndoG in the Tg rats compared with the Wt rats. Less apoptotic cell death was detected in the Tg rats than in the Wt rats 3 days after SCI. These results suggest that translocation of EndoG and AIF from mitochondria to nuclei may initiate a caspase-independent pathway of apoptosis. An increased level of SOD1 in mitochondria conceivably reduces oxidative stress, thereby attenuating EndoG translocation, and resulting in reduction of caspase-independent apoptosis.

• Authors: Fengshan Yu, Taku Sugawara, Tatsuro Nishi, Jing Liu, Pak H Chan
• Journal: Journal of neurotrauma (J Neurotrauma) Vol. 23 Issue 5 Pg. 595-603 (May 2006) ISSN: 0897-7151 [Print] United States
• PMID: 16689664 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Inducing Factor
• Sod1 protein, rat
• Superoxide Dismutase
• Superoxide Dismutase-1
• superoxide dismutase 2
• Endodeoxyribonucleases
• endonuclease G
• Caspases

Topics

• Animals
• Animals, Genetically Modified
• Apoptosis (physiology)
• Apoptosis Inducing Factor (metabolism)
• Blotting, Western
• Caspases (metabolism)
• Cell Nucleus (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Endodeoxyribonucleases (metabolism)
• Female
• Immunohistochemistry
• Mitochondria (metabolism)
• Motor Neurons (metabolism, pathology)
• Oxidative Stress (physiology)
• Protein Transport (physiology)
• Rats
• Spinal Cord Injuries (metabolism, pathology)
• Superoxide Dismutase (metabolism)
• Superoxide Dismutase-1