Early rise in exhaled nitric oxide and mast cell activation in repeated low-dose allergen challenge.

Abstract

Repeated low-dose allergen inhalation challenge mimics natural allergen exposure, providing a model for early mechanisms in the triggering of asthma. The current authors performed a controlled study to evaluate the time course of changes in exhaled nitric oxide fraction (F(e,NO)) and urinary biomarkers of airway inflammation. Eight subjects with mild allergic asthma completed two 7-day repeated low-dose challenge periods, with diluent and allergen, respectively. Subjects were symptom free at inclusion and were investigated when not exposed to specific allergen. Pulmonary function and symptoms were followed, and F(e,NO) and urinary mediators were correlated to changes in airway responsiveness to histamine and adenosine. Despite no change in pulmonary function (forced expiratory volume in one second mean+/-sem fall 0.3+/-0.7 versus 0.6+/-1.0%, for diluent and allergen, respectively) and no asthma symptoms, repeated allergen exposure, in contrast to diluent, caused significant increases in histamine responsiveness (2.3 doubling doses), an early and gradual increase in F(e,NO) (up to a doubling from baseline) and a small increase in the mast cell marker 9alpha11beta-prostaglandin F(2) after adenosine challenge. In conclusion, serial measurements of exhaled nitric oxide fraction have the potential to provide a very sensitive strategy for early detection of emerging airway inflammation and subsequent changes in airway hyperresponsiveness to histamine.

• Authors: E Ihre, P Gyllfors, L E Gustafsson, M Kumlin, B Dahlén
• Journal: The European respiratory journal (Eur Respir J) Vol. 27 Issue 6 Pg. 1152-9 (Jun 2006) ISSN: 0903-1936 [Print] England
• PMID: 16510451 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Leukotrienes
• Prostaglandins
• Nitric Oxide
• Adenosine Monophosphate

Topics

• Adenosine Monophosphate (physiology)
• Administration, Inhalation
• Adult
• Allergens (immunology)
• Animals
• Asthma (diagnosis, immunology)
• Breath Tests
• Cross-Over Studies
• Female
• Forced Expiratory Volume (physiology)
• Humans
• Intradermal Tests
• Leukotrienes (physiology)
• Male
• Mast Cells (immunology)
• Nitric Oxide (physiology)
• Pollen
• Prostaglandins (physiology)
• Reference Values
• Respiratory Hypersensitivity (diagnosis, immunology)