Abstract | BACKGROUND: METHODS AND RESULTS:
LDL receptor (LDLR)(-/-) and apolipoprotein E ( apoE)(-/-) recipient mice were lethally irradiated and transplanted with COX-1(-/-) bone marrow. Mice reconstituted with COX-1(-/-) marrow had nearly complete (99.7%) loss of platelet TXA2 and significantly suppressed levels of macrophage and urinary TXA2 metabolites. Serum lipid levels and lipoprotein distributions did not differ between recipients reconstituted with COX-1(+/+) and COX-1(-/-) marrow. Surprisingly, the extent of atherosclerotic lesions in both LDLR(-/-) and apoE(-/-) mice reconstituted with COX-1(-/-) marrow was increased significantly compared with control mice transplanted with COX-1(+/+) marrow. Peritoneal macrophages isolated from LDLR(-/-) mice reconstituted with COX-1(-/-) marrow had increased lipopolysaccharide-induced levels of COX-2 mRNA and protein expression. Fetal liver cell transplantation studies revealed a 30% increase in atherosclerosis in COX-1(-/-)-->LDLR(-/-)mice compared with COX-1(+/+)-->LDLR(-/-)mice, whereas the extent of atherosclerosis was unchanged in COX-1(-/-)/COX-2(-/-)-->LDLR(-/-)mice. CONCLUSIONS: COX-1 deficiency in bone marrow-derived cells worsens early atherosclerosis in apoE(-/-) and LDLR(-/-) mice despite virtual elimination of platelet TX production. These data demonstrate that platelet TX production does not aggravate early atherosclerotic lesion formation and that upregulation of COX-2 expression in COX-1(-/-) macrophages is proatherogenic.
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Authors | Vladimir R Babaev, Lei Ding, Jeff Reese, Jason D Morrow, Matthew D Breyer, Sudhansu K Dey, Sergio Fazio, MacRae F Linton |
Journal | Circulation
(Circulation)
Vol. 113
Issue 1
Pg. 108-17
(Jan 03 2006)
ISSN: 1524-4539 [Electronic] United States |
PMID | 16380543
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoproteins E
- Receptors, LDL
- Thromboxane A2
- Cyclooxygenase 1
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Topics |
- Animals
- Apolipoproteins E
(deficiency)
- Atherosclerosis
(etiology)
- Bone Marrow Cells
(enzymology)
- Bone Marrow Transplantation
- Cyclooxygenase 1
(deficiency, genetics)
- Mice
- Mice, Knockout
- Mice, Transgenic
- Receptors, LDL
(deficiency)
- Thromboxane A2
(biosynthesis, physiology)
- Up-Regulation
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