Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.

Abstract

A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.

• Authors: Hongbo Hu, Li Li, Richard Y Kao, Binbin Kou, Zhanguo Wang, Liang Zhang, Huiyuan Zhang, Zhiyong Hao, Wayne H Tsui, Anping Ni, Lianxian Cui, Baoxing Fan, Feng Guo, Shuan Rao, Chengyu Jiang, Qian Li, Manji Sun, Wei He, Gang Liu
• Journal: Journal of combinatorial chemistry (J Comb Chem) 2005 Sep-Oct Vol. 7 Issue 5 Pg. 648-56 ISSN: 1520-4766 [Print] United States
• PMID: 16153058 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral
• Epitopes, B-Lymphocyte
• Peptide Library

Topics

• Amino Acid Sequence
• Antigens, Viral (chemistry, genetics, immunology)
• Combinatorial Chemistry Techniques
• Epitopes, B-Lymphocyte (chemistry, genetics, immunology)
• Molecular Sequence Data
• Peptide Library
• Severe acute respiratory syndrome-related coronavirus (chemistry, immunology)
• Severe Acute Respiratory Syndrome (immunology)