Effects of insulin-like growth factor-1 on okadaic acid-induced apoptosis in SH-SY5Y cells.

Abstract

The effects of insulin-like growth factor-1 (IGF-1) on the cytotoxicity and apoptosis induced by okadaic acid (OA) in SH-SY5Y cells were investigated. Cell viability was measured using the MTT (3-(4,5-dimethylthiazolyl-2)-2,-5-diphenyltetrazolium bromide) assay. Early and late apoptosis/necrosis were analyzed by flow cytometry using Annexin V and propidium iodide (PI) double-staining. Caspase-3 activation was detected by Western blot analysis. Preincubation with IGF-1 for 24 h prevented cytotoxicity induced by 40 nM OA given for 24 h, and the MTT value significantly increased. Incubation with 20 nM OA for 24 h caused a marked increase in the percentage of early apoptotic and late apoptotic/necrotic cells, which was not dependent on the activation of caspase-3. OA-induced apoptosis was significantly decreased by pretreatment with 10 ng/ml of IGF-1 for 24 h. The results supported the hypothesis that IGF-1 may be useful in the treatment of Alzheimer's disease.

• Authors: Changhong Xing, Ying Peng, Rui Chang, Yanling Yin, Zuoping Xie
• Journal: Cell biology international (Cell Biol Int) Vol. 29 Issue 9 Pg. 803-8 (Sep 2005) ISSN: 1065-6995 [Print] England
• PMID: 16087362 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Annexin A5
• Enzyme Inhibitors
• Indicators and Reagents
• Okadaic Acid
• Propidium
• Insulin-Like Growth Factor I

Topics

• Annexin A5
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Drug Interactions
• Enzyme Inhibitors (pharmacology)
• Flow Cytometry
• Humans
• Indicators and Reagents
• Insulin-Like Growth Factor I (pharmacology)
• Neurons (drug effects)
• Okadaic Acid (pharmacology)
• Propidium