WDRPUH, a novel WD-repeat-containing protein, is highly expressed in human hepatocellular carcinoma and involved in cell proliferation.

Abstract	In an attempt to disclose mechanisms of hepatocarcinogenesis and discover novel target molecules for the diagnosis and treatment of hepatocellular carcinomas (HCCs), we previously analyzed expression profiles of HCC tissues by means of human cDNA microarray. Among the genes upregulated in tumor tissues compared with their nontumor counterparts, we focused on a novel gene, termed WDRPUH, and characterized its biologic function. WDRPUH encodes a predicted 620-amino acid protein containing 11 highly conserved WD40-repeat domains. Multiple-tissue Northern blot analysis revealed its specific expression in the testis among 16 normal tissues examined. Transfection of plasmids designed to express WDRPUH-specific siRNA significantly reduced its expression in HCC cells and resulted in growth suppression of transfected cells. Interestingly, we found that WDRPUH associated with HSP70, proteins of the chaperonin-containing TCP-1 (CCT1) complex, as well as BRCA2. These findings have disclosed a novel insight into hepatocarcinogenesis and suggested that WDRPUH may be a molecular target for the development of new strategies to treat HCCs.
Authors	Fabio Pittella Silva, Ryuji Hamamoto, Yusuke Nakamura, Yoichi Furukawa
Journal	Neoplasia (New York, N.Y.) (Neoplasia) Vol. 7 Issue 4 Pg. 348-55 (Apr 2005) ISSN: 1522-8002 [Print] United States
PMID	15967112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	BRCA2 Protein CFAP52 protein, human Carrier Proteins DNA, Complementary HSP70 Heat-Shock Proteins RNA, Small Interfering RNA Chaperonins
Topics	Animals BRCA2 Protein (metabolism) Blotting, Northern Carcinoma, Hepatocellular (metabolism) Carrier Proteins (biosynthesis, chemistry) Cell Line Cell Line, Tumor Cell Proliferation Cell Survival Chaperonins (chemistry) Cilia Cloning, Molecular DNA, Complementary (metabolism) Gene Expression Regulation, Neoplastic Gene Silencing HSP70 Heat-Shock Proteins (metabolism) Humans Immunoblotting Immunoprecipitation Liver Neoplasms (metabolism, pathology) Mass Spectrometry Mice Microscopy, Fluorescence NIH 3T3 Cells Oligonucleotide Array Sequence Analysis Plasmids (metabolism) Protein Binding Protein Structure, Tertiary RNA (metabolism) RNA, Small Interfering (metabolism) Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tissue Distribution Transfection Up-Regulation

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