Abstract | BACKGROUND AND AIMS: MATERIALS AND METHODS: Seventy-five male Sprague-Dawley rats were randomized into five groups (gr.) (n=15). Gr. 1 underwent a laparotomy followed by saline infusion, gr. 2-5 received intraductal glycodeoxycholic acid (GDOC) followed by intravenous cerulein. Six hours after induction of pancreatitis (IOP), gr. 2 received saline infusion, while gr. 3 was infused with standard lipovenous (rich in [n-6] polyunsaturated fatty acids (PUFA)), gr. 4 received ClinOleic (rich in [n-9] PUFA), while gr. 5 was infused with Omegaven (rich in [ n-3] PUFA) for 18 h. After 24 h, all animals were sacrificed and the pancreas was determined histopathologically according to the severity of pancreatitis. Furthermore, pancreatic lipidperoxidation ( TBARS) and activity of lipid production protective enzymes superoxide dismutase (SOD) and gluthationperoxidase (GSHPx) were analyzed. RESULTS:
Omegaven infusion reduced the severity of histopathologic changes in acute pancreatitis and decreased lipidperoxidation ( TBARS) in pancreatic tissue samples. Furthermore, pancreatic activity of SOD was increased. However, standard PUFA and ClinOleic infusion did not influence the severity of pancreatitis and lipidperoxidation. CONCLUSION:
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Authors | M Kilian, I Heukamp, J I Gregor, C Bretthauer, M K Walz, C A Jacobi, H Lochs, I Schimke, H Guski, F A Wenger |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 21
Issue 1
Pg. 57-63
(Jan 2006)
ISSN: 0179-1958 [Print] Germany |
PMID | 15864609
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Fatty Acids, Omega-3
- Fatty Acids, Omega-6
- Fatty Acids, Unsaturated
- Thiobarbituric Acid Reactive Substances
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Topics |
- Animals
- Disease Models, Animal
- Fatty Acids, Omega-3
(pharmacology)
- Fatty Acids, Omega-6
(pharmacology)
- Fatty Acids, Unsaturated
(pharmacology)
- Immunohistochemistry
- Infusions, Intravenous
- Lipid Peroxidation
(drug effects)
- Male
- Pancreatitis, Acute Necrotizing
(drug therapy, mortality, pathology)
- Parenteral Nutrition, Total
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Sensitivity and Specificity
- Survival Rate
- Thiobarbituric Acid Reactive Substances
(metabolism)
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