The effect of interleukin-10 on acute pancreatitis induced by cerulein in a rat experimental model.

Abstract

To investigate whether interleukin-10, a potent anti-inflammatory cytokine, could have a therapeutic effect on rats on that were made pancreatitis by cerulein. Thirty Wistar Albino rats were randomized into sham, pancreatitis, and therapy groups (n = 10 in each). Nothing was applied to the sham group; pancreatitis by inject-ing cerulein (50 micro/g/kg/h) was induced in the pancreatitis and therapy groups. Interleukin-10 (10.000 U) was injected at 1 and 4 h after pancreatitis inductions in the therapy group. The rats were sacrificed at postoperative hour 24. The following parameters were investigated: the leukocyte count, blood glucose, amylase, lipase and tumor necrosis factor-alpha levels in the blood samples; histopathological search, and wet/dry weight ratios of the pancreas tissues. The ratio of wet/dry pancreatic tissue weight, serum tumor necrosis factor-alpha, amylase and lipase lev-els, and histologic damage scores in the pancreatitis and therapy groups were significantly higher when they were compared with the sham group(p < .01). However, all of these values were significantly lower in the therapy groups than in the pancreatitis group (p < .01). Interleukin-10 decreases pancreatic tissue injury induced by cerulein-induced pancreatitis in rats. Nevertheless, more experimental studies are needed to compare endogenous interleukin-10 with exogenous interleukin-10 effects before clinical usage of this drug.

• Authors: Mustafa Keceli, Can Kucuk, Erdogan Sozuer, Mustafa Kerek, Ozhan Ince, Makbule Arar
• Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (J Invest Surg) 2005 Jan-Feb Vol. 18 Issue 1 Pg. 7-12 ISSN: 0894-1939 [Print] United States
• PMID: 15804946 (Publication Type: Journal Article)

Chemical References

• Tumor Necrosis Factor-alpha
• Interleukin-10
• Ceruletide

Topics

• Acute Disease
• Animals
• Ceruletide
• Disease Models, Animal
• Interleukin-10 (pharmacology)
• Male
• Organ Size
• Pancreas (pathology)
• Pancreatitis (chemically induced, drug therapy, pathology)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (metabolism)