Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO.

Abstract

AIM:

To investigate the signal pathway of honokiol-induced apoptosis on human colorectal carcinoma RKO cells and to evaluate whether p53 and p53-related genes were involved in honokiol-treated RKO cells.

METHODS:

Cell cycle distribution and subdiploid peak were analyzed with a flow cytometer and DNA fragment with electrophoresis on agarose gels. Transcriptional level of Bax, Bcl-2, Bid and Bcl-xl was accessed by RT-PCR. Western blotting was used to measure p53 protein expression and other factors related to apoptosis. Proliferation inhibition of two cell lines (RKO, SW480) with high expression of p53 and one cell line with p53 negative expression (LS180) was monitored by MTT assay.

RESULTS:

Honokiol induced RKO cell apoptosis in a dose-dependent manner. The mRNA expression level and protein level of Bid were up-regulated while that of Bcl-xl was down-regulated, but no changes in Bax and Bcl-2 were observed. Western blotting showed p53 expression had no remarkable changes in honokiol-induced RKO cell apoptosis. LS180 cells treated with honokiol exhibited apparent growth inhibition like RKO cells and Sw480 cells.

CONCLUSION:

Honokiol can induce RKO cells apoptosis through activating caspase cascade by p53-indepenent pathway.

• Authors: Tao Wang, Fei Chen, Zhe Chen, Yi-Feng Wu, Xiao-Li Xu, Shu Zheng, Xun Hu
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 10 Issue 15 Pg. 2205-8 (Aug 01 2004) ISSN: 1007-9327 [Print] United States
• PMID: 15259066 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Biphenyl Compounds
• Lignans
• Tumor Suppressor Protein p53
• honokiol

Topics

• Antineoplastic Agents, Phytogenic (therapeutic use)
• Apoptosis (drug effects)
• Biphenyl Compounds (therapeutic use)
• Cell Line, Tumor
• Colorectal Neoplasms (drug therapy, physiopathology)
• Humans
• Lignans
• Tumor Suppressor Protein p53 (metabolism)