Sepharose (Agarose)

04/15/2010 - "In the present study, a tumor collective from Middle Germany was analyzed using two HPV-detection methods, i.e., PCR followed by agarose gel electrophoresis and microarray chip hybridization. "
10/01/2006 - "The samples used in the study included 4T1 and B16.F10 tumors, mouse skin, and tissue phantoms constructed with 1% agarose gel with or without 4T1 cells. "
03/15/1995 - "To elucidate the degree to which these in vivo alterations are a result of intrinsic cellular changes versus radiation-induced systemic effects, the Radiation-Induced Fibrosarcoma-1 tumor model was studied before and over the course of 7 days after a single dose of 17 Gy. In vivo studies were performed with tumors implanted in C3H/He mice; in vitro studies used cells that were perfused in agarose gel threads after being grown, radiated, and maintained in monolayer. "
01/01/1985 - "In the present study, we describe conditions for the assay of human tumor cell movement utilizing agarose microdroplets. "
07/01/1981 - "[Studies on tumor-specific immune response evaluated by leucocyte migration inhibition test under agarose-plate in gynecological malignancy (author's transl)]."

02/01/2015 - "Mice inoculated with PA-laden agarose beads developed chronic PA lung infection during 7-day study period, while mice inoculated with planktonic PA cleared bacteria in 3 days. "
01/01/2015 - "Replication intermediates were displayed in the time course of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization. "
09/01/2013 - "In addition, PHEV caused DNA fragmentation detectable by agarose gel electrophoresis 48h post-infection, increasing with the incubation time. "
12/01/2011 - "Agarose gel electrophoresis detected lesser number of PmDNV infection which may due to the limit of sensitivity. "
06/01/2009 - "In situ immune infrared fluorescent staining techniques were developed to visualize and quantitate BTV infection in Culicoides cell culture by both an endpoint titration and an agarose overlay fluorescent focus assay. "

09/01/1994 - "In 173 patients with various carcinomas, in vitro sensitivity to 7 drugs in the MTT assay in agarose was significantly greater than that in the MTT assay. "
07/01/2002 - "The efficacy of agarose in preventing VX2 carcinoma cell leakage was evaluated and the results were compared with two traditional methods. "
04/01/1986 - "[Experimental study of soft agarose colony formation assay of renal carcinoma]."
09/01/2006 - "Value of the innovated technique agarose cell block in improving the sensitivity of urine cytology in cases of bladder carcinoma."
05/01/2005 - "Labeled single carcinoma cells, distributed in an agarose gel in a monodisperse layer as controlled by light microscopy, became detectable as punctuate signal extinctions when using a small circularly polarized surface coil in conjunction with a T(2)*-weighted GE sequence at 3 T. "

01/01/2014 - "To establish a typical tumor model of ovarian cancer which may be more representative and reliable than traditional monolayer culture and pellet, agarose was used as cell vehicle to engineering tumor. "
01/01/2014 - "In this study, ovarian cancer cell line SKOV3 was encapsulated in agarose hydrogel with cell aggregates and two-dimensional culture as controls. "
01/01/2014 - "In vitro ovarian cancer model based on three-dimensional agarose hydrogel."
04/01/2010 - "A lectin with strong mitogenic activity towards human peripheral blood mononuclear cells (PBMCs) and cytotoxic effect on human ovarian cancer cells has been purified from the mycelium of a phytopathogenic fungus, Rhizoctonia bataticola, using ion exchange chromatography and affinity chromatography on asialofetuin-Sepharose. "
01/01/1986 - "A macrophage migration inhibition factor (OC-MIF) has been isolated from ascites fluid of ovarian cancer patients by affinity chromatography on L-fucose-Sepharose 6B, and characterized biochemically. "

03/13/1986 - "Monoclonal antibody was detected in the ascites fluids by agarose gel electrophoresis. "
07/01/1988 - "For in vitro studies cultured HEY cells were assayed for protein synthesis and plated in agarose to form colonies, and for in vivo studies they were injected intraperitoneally (i.p.) into BALB/c nu/nu (nude) mice at a challenge dose (3 X 10(5) cells) which produced carcinomatosis with ascites, leading to death 30 days following injection. "
01/01/1978 - "The microsomal fraction was isolated from homogenate of 125I-labeled leukemia L 1210 ascites cells by filtration of postmitochondrial supernatant through a Sepharose 4 B column. "
11/01/1999 - "Ascites cells from 15 of 27 or 56% of patients tested bound biotinylated MIS (MIS-biotin) and, of the 11 that grew in soft agarose, 9 of 11 or 82% showed statistically significant inhibition of colony formation. "
12/01/1997 - "Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose."