Wine polyphenols induce hypotension, and decrease cardiac reactivity and infarct size in rats: involvement of nitric oxide.

Abstract

1. The effects of short-term oral administration of red wine polyphenolic compounds (RWPC, 20 mg x kg(-1) day(-1) for 7 days) on haemodynamics, ex vivo cardiac responsiveness and ischaemia-reperfusion injury were investigated in rats. The involvement of nitric oxide (NO) was evaluated using the NO synthase inhibitor, N(G)-nitro-l-arginine methyl ester (l-NAME, 2 mg x kg(-1) day(-1) for 7 days), at a dose which did not affect blood pressure. 2. Ex vivo reactivity of hearts from RWPC-treated rats showed lower basal developed pressure, greater heart rate and decreased inotropic responses to either beta-adrenoceptor or muscarinic receptor stimulation with isoprenaline or carbachol, respectively.3. RWPC treatment did not modify cardiac expression of endothelial NO synthase or Cu/Zn superoxide dismutase. However, it increased nitrite in the coronary effluent. 4. In ischaemia-reperfusion, RWPC treatment reduced infarct size and oxidative stress, as shown by the myocardial content of the end products of lipid peroxidation, malondialdehyde and 4-hydroxynonenal, without affecting post-ischaemic contractile dysfunction. All the observed effects of RWPC were prevented by l-NAME treatment. 5. Altogether, these data show that short-term treatment with RWPC decreases blood pressure and cardiac responsiveness, and protects against post-ischaemic infarction via decreased oxidative stress. All the above effects of RWPC are sensitive to NO synthase inhibition that implies an involvement of NO-dependent pathway. This study suggests a basis for the beneficial effects of plant-derived polyphenols against cardiovascular disease.

• Authors: Hantamalala Ralay Ranaivo, Myriam Diebolt, Ramaroson Andriantsitohaina
• Journal: British journal of pharmacology (Br J Pharmacol) Vol. 142 Issue 4 Pg. 671-8 (Jun 2004) ISSN: 0007-1188 [Print] England
• PMID: 15159281 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aldehydes
• Flavonoids
• Nitrites
• Phenols
• Polyphenols
• Nitric Oxide
• Malondialdehyde
• Carbachol
• 4-hydroxy-2-nonenal
• Isoproterenol
• NG-Nitroarginine Methyl Ester

Topics

• Administration, Oral
• Aldehydes (chemistry, metabolism)
• Animals
• Blood Pressure (drug effects)
• Blotting, Western (methods)
• Carbachol (antagonists & inhibitors, pharmacology)
• Coronary Circulation (drug effects, physiology)
• Drug Administration Schedule
• Drug Therapy, Combination
• Flavonoids (analysis, antagonists & inhibitors, pharmacology)
• France
• Heart Rate (drug effects, physiology)
• Heart Ventricles (chemistry, drug effects)
• Hypotension (chemically induced, prevention & control)
• Isoproterenol (pharmacology)
• Male
• Malondialdehyde (chemistry, metabolism)
• Myocardial Infarction (diagnosis, drug therapy, prevention & control)
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide (analysis, metabolism)
• Nitrites (analysis, metabolism)
• Phenols (analysis, antagonists & inhibitors, pharmacology)
• Polyphenols
• Rats
• Rats, Wistar
• Reperfusion Injury (diagnosis, drug therapy, prevention & control)
• Time Factors
• Ventricular Function
• Ventricular Pressure (drug effects, physiology)
• Wine (analysis)