Vaccination with recombinant whole heavy chain fragments of Clostridium botulinum Type C and D neurotoxins.

Abstract

Mice and ducks were subcutaneously immunized with recombinant whole heavy (H) chains of Clostridium botulinum type C and D neurotoxins, which were expressed as glutathione S-transferase fusion proteins. In the case of mice, it was confirmed that two immunizations with type C- and D-H chains, 10 microg each time, significantly increased the specific antibodies against 100-kDa H chains of type C and D neurotoxins in an immunoblot analysis and an enzyme-linked immunosorbent assay, respectively. The mice immunized with type C- and D-H chains showed no symptoms of botulism when they were challenged with C- and D-16 S toxins at doses, given intraperitoneally, of up to 10(5) and 10(6) minmum lethal doses (MLD), respectively, per mouse. Ducks were immunized with a total of 100 microg of type C-H chain. The ducks also developed specific antibodies to the type C-H chain and showed significant protection against a challenge with 10(3) duck MLD of C-16 S toxin given intravenously. These results indicate that recombinant whole H chains can be used as an effective and safe vaccine for type C and D botulism in domestic animals.

• Authors: Hideyuki Arimitsu, Jae-Chul Lee, Yoshihiko Sakaguchi, Yuji Hayakawa, Michiko Hayashi, Miki Nakaura, Hikaru Takai, Song-Nan Lin, Masafumi Mukamoto, Tom Murphy, Keiji Oguma
• Journal: Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 11 Issue 3 Pg. 496-502 (May 2004) ISSN: 1071-412X [Print] United States
• PMID: 15138174 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Protein Subunits
• Recombinant Fusion Proteins
• botulinum toxin type D
• Botulinum Toxins
• botulinum toxin type C

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Bacterial (blood)
• Antibody Specificity
• Blotting, Western
• Botulinum Toxins (genetics, immunology, pharmacology)
• Botulism (prevention & control)
• Clostridium botulinum (chemistry, genetics)
• Ducks
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Escherichia coli (genetics)
• Female
• Male
• Mice
• Molecular Sequence Data
• Plasmids (genetics)
• Protein Subunits (administration & dosage, genetics, immunology)
• Recombinant Fusion Proteins (administration & dosage, immunology, isolation & purification)
• Sequence Homology, Amino Acid
• Transfection
• Vaccination