3-Hydroxypropionic acid and methylcitric acid are not reliable indicators of marginal biotin deficiency in humans.
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| Abstract |
In two studies comprising 10 and 11 subjects, respectively, marginal biotin deficiency was induced experimentally by an egg-white diet in healthy men and women. The following urinary organic acids were assessed for their usefulness in detecting marginal biotin status: 1) 3-hydroxypropionic acid and methylcitric acid, organic acids that reflect decreased activity of the biotin-dependent enzyme propionyl-CoA carboxylase and 2) methylcrotonylglycine and isovalerylglycine, organic acids that reflect decreased activity of methylcrotonyl-CoA carboxylase. Mean 3-hydroxypropionic acid excretion rates remained normal during biotin depletion in both studies. By the end of the depletion period, 3-hydroxypropionic acid excretion identified only 5 of 21 marginally deficient subjects. Mean methylcitric acid excretion increased (P < 0.0001) in the first study but not in the second. Mean methylcrotonylglycine excretion increased in each study (P < 0.004 and P < 0.05, respectively); methylcrotonylglycine excretion identified 13 of 21 marginally deficient subjects. Mean isovalerylglycine excretion increased only in the first study (P = 0.006) and identified only 6 of 21 deficient subjects. We conclude that none of these organic acids is as sensitive an indicator of marginal biotin deficiency as 3-hydroxyisovaleric acid, which reflects decreased methylcrotonyl-CoA carboxylase.
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| Authors | Donald M Mock, Cindy L Henrich-Shell, Nadine Carnell, Phyllis Stumbo, Nell I Mock |
| Journal | The Journal of nutrition (J Nutr) Vol. 134 Issue 2 Pg. 317-20 (Feb 2004) ISSN: 0022-3166 [Print] United States |
| PMID | 14747666 (Publication Type: Clinical Trial, Journal Article, Research Support, U.S. Gov't, P.H.S.) |
| Chemical References |
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