Abstract |
Neuronal apoptosis may contribute to pathologic neuronal loss in certain disease states such as neurodegenerative diseases. Staurosporine (ST), a nonselective protein kinase inhibitor, has been shown to induce apoptosis in a variety of cells including nerve cell lines. In this study, we investigated the neuroprotective effect of sauchinone, which is a unique lignan from Saururus chinensis, on ST-induced apoptosis in C6 rat glioma cells. Sauchinone attenuated ST-induced apoptosis of C6 glioma cells as evidenced by DNA fragmentation. We also provide evidence that the inhibitory effect of sauchinone on ST-induced apoptosis involves a dose-dependent upregulation of an antiapoptotic protein, Bcl-2. Mounting evidence shows that the activation of caspases, especially caspase-3, triggers the apoptotic process. The activity of caspase-3 of ST-pretreated cells was significantly decreased upon sauchinone treatment in a dose-dependent manner. Taken together, the data demonstrate that sauchinone protects C6 glioma cells from ST-induced apoptosis in a caspase-3 dependent manner. Our findings may be critical for developing a strategy to protect nerve cells from apoptosis, suggesting the potential development of sauchinone as a neuroprotective agent.
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Authors | Hyun Song, Young Choong Kim, Aree Moon |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 26
Issue 10
Pg. 1428-30
(Oct 2003)
ISSN: 0918-6158 [Print] Japan |
PMID | 14519949
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzopyrans
- Dioxoles
- Lignans
- sauchinone
- Staurosporine
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Topics |
- Animals
- Apoptosis
(drug effects, physiology)
- Benzopyrans
(chemistry, pharmacology)
- Cell Line, Tumor
(drug effects, physiology)
- DNA Fragmentation
(drug effects, physiology)
- Dioxoles
(chemistry, pharmacology)
- Dose-Response Relationship, Drug
- Lignans
(chemistry, pharmacology)
- Rats
- Saururaceae
- Staurosporine
(pharmacology)
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