The calibrated automated thrombogram (CAT): a universal routine test for hyper- and hypocoagulability.

Abstract

By using a "slow" fluorogenic thrombin substrate and continuous comparison to a simultaneously run calibrator, thrombin generation can be monitored automatically, on line, in clotting PPP or PRP at a throughput of up to 100 samples per hour. The resulting "Thrombogram" in PPP measures hypocoagulability (haemophilias, oral anticoagulants, heparins (-likes), direct inhibitors) and hypercoagulabilities (AT deficiency, prothrombin hyperexpression, prot. C and S deficiency, factor V Leiden, oral contraceptives). In PRP it is diminished in thrombopathies, in von Willebrand disease, by antibodies blocking GPIIb-IIIa or GPIb, or by antiplatelet drugs like aspirin and clopidogrel. Lupus anticoagulant both retards and increases thrombin generation. The thrombogram thus appears to be a broad function test of the haemostatic-thrombotic mechanism of the blood.

• Authors: H C Hemker, P Giesen, R AlDieri, V Regnault, E de Smed, R Wagenvoord, T Lecompte, S Béguin
• Journal: Pathophysiology of haemostasis and thrombosis (Pathophysiol Haemost Thromb) 2002 Sep-Dec Vol. 32 Issue 5-6 Pg. 249-53 ISSN: 1424-8832 [Print] Switzerland
• PMID: 13679651 (Publication Type: Journal Article, Review)

Chemical References

• Thrombin

Topics

• Blood Coagulation Disorders (diagnosis)
• Blood Coagulation Tests (methods)
• Humans
• Thrombin (analysis)