[All-trans retinoic acid augments the bystander effect of herpes simplex virus thymidine kinase/ganciclovir system in the treatment of tongue carcinoma cell line].

Abstract

OBJECTIVE:

To investigate the augmentation effect and mechanism of all-trans retinoic acid (ATRA) on the bystander effect of herpes simplex virus thymidine kinase (HSV-TK)/ganciclovir (GCV) system in the treatment of tongue carcinoma cell line.

METHODS:

Immunocytochemistry and flow cytometry (FCM) were used to analyze the expression of Cx43 protein in Tca8113 cells after treated with ATRA; The bystander effect of HSV-TK/GCV system on tongue carcinoma cells before and after treatment with ATRA was detected by MTT assays. The interaction of ATRA and bystander effect was analyzed by factorial experiment.

RESULTS:

After treated by ATRA (10(-7) mol/L - 10(-5) mol/L), the expression of Cx43 protein was up-regulated in Tca8113 cells and the positive rate of Cx43 protein increased from 5.17% (before treatment) to 30.53% (10(-5) mol/L ATRA). There was significant difference between ATRA treated cells and untreated cells (P < 0.01). The bystander effect of HSV-TK/GCV system on Tca8113 cells was poor, but improved after combined with ATRA. There was cooperation effect between ATRA and bystander effect of HSV-TK/GCV (P < 0.05).

CONCLUSIONS:

ATRA can augment the bystander effect of HSV-TK/GCV system in the treatment of tongue carcinoma cell line. The mechanism might be due to up-regulation of Cx43 gene and restore gap junction intercellular communication.

• Authors: An-xun Wang, Hong-zhang Huang
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 38 Issue 1 Pg. 24-6 (Jan 2003) ISSN: 1002-0098 [Print] China
• PMID: 12760771 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Connexin 43
• Tretinoin
• Thymidine Kinase
• Ganciclovir

Topics

• Antineoplastic Agents (pharmacology)
• Bystander Effect (drug effects)
• Cell Survival (drug effects)
• Connexin 43 (metabolism)
• Dose-Response Relationship, Drug
• Drug Synergism
• Ganciclovir (pharmacology)
• Humans
• Simplexvirus (enzymology)
• Thymidine Kinase (genetics)
• Tongue Neoplasms (metabolism, pathology, therapy)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured (drug effects, metabolism)