Abstract | BACKGROUND: METHODS: A human pancreatic carcinoma cell line, SUIT-2, was incubated with various concentrations of curcumin for 2 hours. Biologic features, including IL-8 production, DNA binding activity, transactivation of NF-kappaB, cell growth activity, cell viability, and the expression of IL-8 receptors (CXCR1 and CXCR2) were analyzed. RESULTS: The constitutive production of IL-8 was inhibited by curcumin at concentrations of 10-100 microM in a dose dependent manner. NF-kappaB activity was reduced significantly by curcumin treatment. Pretreatment with curcumin inhibited the growth rate of carcinoma cells significantly. Such cell growth inhibition by curcumin was not recovered by exogenous recombinant IL-8. The investigation of expression in IL-8 receptors, CXCR1 and CXCR2, revealed that the expression of both receptors was enhanced remarkably by curcumin. Exogenous IL-8 could not recover this enhancement of IL-8 receptors. These results suggest that curcumin inhibits IL-8-induced receptor internalization. CONCLUSIONS:
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Authors | Hideki Hidaka, Takatoshi Ishiko, Takashi Furuhashi, Hidenobu Kamohara, Shunji Suzuki, Masashi Miyazaki, Osamu Ikeda, Seiji Mita, Toshiaki Setoguchi, Michio Ogawa |
Journal | Cancer
(Cancer)
Vol. 95
Issue 6
Pg. 1206-14
(Sep 15 2002)
ISSN: 0008-543X [Print] United States |
PMID | 12216086
(Publication Type: Journal Article)
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Copyright | Copyright 2002 American Cancer Society. |
Chemical References |
- Antineoplastic Agents
- Interleukin-8
- Receptors, Interleukin-8A
- Curcumin
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Topics |
- Antineoplastic Agents
(administration & dosage, pharmacology)
- Cell Division
(drug effects)
- Cell Line
- Curcumin
(administration & dosage, pharmacology)
- Dose-Response Relationship, Drug
- Down-Regulation
- Flow Cytometry
- Humans
- Interleukin-8
(biosynthesis)
- Pancreatic Neoplasms
(metabolism, pathology)
- Receptors, Interleukin-8A
(analysis)
- Signal Transduction
(drug effects)
- Tumor Cells, Cultured
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