Abstract | OBJECTIVE: RESEARCH METHODS AND PROCEDURES: We developed an anti-idiotypic polyclonal antibody against the region of a leptin monoclonal antibody that competitively binds leptin, mimicking the active site structure of leptin. To test whether our anti-idiotype could also reproduce leptin functions, we examined food intake, body weight, and colonic temperature in male Wistar rats (n = 9) in response to intracerebroventricular administration of the leptin anti-idiotype. RESULTS: Our leptin anti-idiotype induced a significant reduction in food intake coupled with an increase in body temperature comparable to that of leptin. That is, the intracerebroventricular administration of 8.0 microg of leptin anti-idiotype or 5.0 microg leptin significantly increased colonic temperature (Delta 1.9 +/- 0.11 degrees C and Delta 1.7 +/- 0.12 degrees C, respectively). In addition, both decreased 24-hour food intake (-26.4 +/- 2.4% and -21.9 +/- 2.2%) compared with the control. The gain in body weight was also decreased by acute administration of the anti-idiotype (-1.4 +/- 0.28%) and leptin (-1.1 +/- 0.17%) vs. the phosphate-buffered saline control (1.3 +/- 0.15%). DISCUSSION: These studies revealed that the leptin anti-idiotype inhibited food intake and enhanced heat production, mimicking leptin's central actions.
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Authors | Brant A De Fanti, Fermin I Milagro, Oscar Lamas, Eduardo Martínez-Ansó, J Alfredo Martínez |
Journal | Obesity research
(Obes Res)
Vol. 10
Issue 8
Pg. 833-7
(Aug 2002)
ISSN: 1071-7323 [Print] United States |
PMID | 12181393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Anti-Idiotypic
- Antibodies, Monoclonal
- Leptin
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Topics |
- Animals
- Antibodies, Anti-Idiotypic
(administration & dosage, pharmacology)
- Antibodies, Monoclonal
(immunology)
- Appetite
(drug effects)
- Body Temperature
(drug effects)
- Body Weight
(drug effects)
- Colon
- Eating
(drug effects)
- Injections, Intraventricular
- Leptin
(immunology, physiology)
- Male
- Rats
- Rats, Wistar
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