Sodium channel expression in hypothalamic osmosensitive neurons in experimental diabetes.

Abstract

Vasopressin is synthesized by neurons in the supraoptic nucleus of the hypothalamus and its release is controlled by action potentials produced by specific subtypes of voltage-gated sodium channels expressed in these neurons. The hyperosmotic state associated with uncontrolled diabetes mellitus causes elevated levels of plasma vasopressin, which are thought to contribute to the pathologic changes of diabetic nephropathy. We demonstrate here that in the rodent streptozotocin model of diabetes there are increases in expression of mRNA and protein for two sodium channel alpha-subunits and two beta-subunits in the neurons of the supraoptic nucleus. Transient and persistent sodium currents show parallel increases in these diabetic neurons. In the setting of chronic uncontrolled diabetes, these changes in sodium channel expression in the supraoptic nucleus may be maladaptive, contributing to the development of secondary renal complications.

• Authors: Joshua P Klein, Matthew J Craner, Theodore R Cummins, Joel A Black, Stephen G Waxman
• Journal: Neuroreport (Neuroreport) Vol. 13 Issue 11 Pg. 1481-4 (Aug 07 2002) ISSN: 0959-4965 [Print] England
• PMID: 12167778 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• NAV1.2 Voltage-Gated Sodium Channel
• Nerve Tissue Proteins
• RNA, Messenger
• Scn2A protein, rat
• Sodium Channels

Topics

• Animals
• Diabetes Mellitus, Experimental (metabolism)
• Male
• NAV1.2 Voltage-Gated Sodium Channel
• Nerve Tissue Proteins (biosynthesis, genetics)
• Neurons (metabolism)
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Sprague-Dawley
• Sodium Channels (biosynthesis, genetics)
• Supraoptic Nucleus (metabolism)
• Up-Regulation (physiology)
• Water-Electrolyte Balance (physiology)