Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome.
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| Abstract |
We are studying peptide immunogenicity as a function of the similarity level to the host's proteome. By using as a model the breast/prostate cancer-associated HER-2/neu antigen, we analyzed the monoclonal and polyclonal humoral immune responses against HER-2/neu peptide motifs not shared with the host proteome. We show here that (i) a mouse monoclonal antibody (MAb) raised against the extracellular domain (EC) of human HER-2/neu oncoprotein recognized a linear peptide motif endowed with low similarity level to the mouse proteome; (ii) likewise, human sera from breast/prostate cancer patients preferentially recognized peptide fragments from the EC of the HER-2/neu oncoprotein having sequences that are not present in the human proteome. Together with previous results obtained in other disease models (cervical cancer-associated HPV16 E7 oncoprotein and Pemphigus vulgaris auto-antigen desmoglein-3), the present data suggest that a low level of sequence similarity to the host's proteome might be an important factor in shaping the pool of B cell epitopes.
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| Authors | Abraham Mittelman, Alberta Lucchese, Animesh A Sinha, Darja Kanduc |
| Journal | International journal of cancer (Int J Cancer) Vol. 98 Issue 5 Pg. 741-7 (Apr 10 2002) ISSN: 0020-7136 [Print] United States |
| PMID | 11920645 (Publication Type: Comparative Study, Journal Article) |
| Copyright | Copyright 2002 Wiley-Liss, Inc. |
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