Abstract | BACKGROUND AND AIMS:
Obesity increases the risk of colon cancer, whereas physical activity reduces the risk. Plasma levels of leptin increase in proportion to the level of obesity and are reduced by physical activity. Leptin acts as a growth factor for several cell types and thus may provide a biological explanation for the observed epidemiological risk factors. The aim of this study was to investigate whether leptin is a growth factor for colonic epithelial cells. METHODS: The presence of the leptin receptor in human colon cancer cell lines was assessed using reverse-transcription polymerase chain reaction and immunoblotting, and its presence in human colonic tissue was assessed by immunohistochemistry. The effects of leptin in vitro on HT29 cells were assessed by assessing p42/44 mitogen-activated protein kinase phosphorylation, thymidine incorporation, and cell numbers and in vivo in C57BL/6 mice by colonic bromodeoxyuridine incorporation. RESULTS: CONCLUSIONS:
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Authors | J C Hardwick, G R Van Den Brink, G J Offerhaus, S J Van Deventer, M P Peppelenbosch |
Journal | Gastroenterology
(Gastroenterology)
Vol. 121
Issue 1
Pg. 79-90
(Jul 2001)
ISSN: 0016-5085 [Print] United States |
PMID | 11438496
(Publication Type: Journal Article)
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Chemical References |
- Carrier Proteins
- Growth Substances
- LEPR protein, human
- Leptin
- Receptors, Cell Surface
- Receptors, Leptin
- leptin receptor, mouse
- Protein Kinases
- Bromodeoxyuridine
- Thymidine
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Topics |
- Adenocarcinoma
(etiology, metabolism, pathology)
- Animals
- Bromodeoxyuridine
(metabolism)
- Carrier Proteins
(drug effects, isolation & purification)
- Colonic Neoplasms
(etiology, metabolism, pathology)
- Epithelium
(drug effects, metabolism)
- Female
- Growth Substances
(pharmacology)
- Humans
- Leptin
(isolation & purification, pharmacology)
- Mice
- Obesity
(complications)
- Protein Kinases
(metabolism)
- Receptors, Cell Surface
- Receptors, Leptin
- Reverse Transcriptase Polymerase Chain Reaction
- Thymidine
(metabolism)
- Tumor Cells, Cultured
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