Cytokine response to human liver ischemia-reperfusion injury during hepatectomy: marker of injury or surgical stress?

Abstract

BACKGROUND/AIMS:

The aim of this study was to evaluate the inflammatory or antiinflammatory cytokine response to ischemia-reperfusion during hepatectomy and to find a useful marker of injury or surgical stress during hepatic ischemia-reperfusion.

METHODOLOGY:

In 9 patients with liver disease who underwent hepatectomy using the Pringle maneuver, serum cytokines, including alanine transaminase, aspartate transaminase, and hyaluronic acid, were measured just prior to vascular occlusion; 5, 10 and 15 min after initial clamping; and 3 min after initial declamping.

RESULTS:

The mean concentrations of aspartate transaminase and alanine transaminase did not significantly differ before and after ischemia-reperfusion during hepatectomy. However, mean concentrations of hyaluronic acid after ischemia-reperfusion were significantly (P < 0.03) higher than before clamping. Although there were no significant differences in the mean concentrations of IL-1 beta, IL-6, IL-8, IL-10 and TNF-alpha among, before and after ischemia-reperfusion, the mean concentrations of granulocyte colony-stimulating factor after ischemia-reperfusion and macrophage colony-stimulating factor after reperfusion were significantly (P < 0.05) higher than before clamping.

CONCLUSIONS:

Although hepatic parenchymal cell function was maintained after ischemia-reperfusion during hepatectomy, sinusoidal endothelial cell dysfunction was found. Release of granulocyte colony-stimulating factor and macrophage colony-stimulating factor after ischemia-reperfusion were also found. These cytokines and hyaluronic acid may be useful indicators in the early phase of human ischemia-reperfusion injury during hepatectomy.

• Authors: K Hanazaki, T Monma, M Hiraguri, Y Ohmoto, S Kajikawa, A Matsushita, Y Nimura, N Koide, W Adachi, J Amano
• Journal: Hepato-gastroenterology (Hepatogastroenterology) 2001 Jan-Feb Vol. 48 Issue 37 Pg. 188-92 ISSN: 0172-6390 [Print] Greece
• PMID: 11268962 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Cytokines
• Interleukins
• Tumor Necrosis Factor-alpha
• Granulocyte Colony-Stimulating Factor
• Macrophage Colony-Stimulating Factor
• Hyaluronic Acid
• Aspartate Aminotransferases
• Alanine Transaminase

Topics

• Aged
• Aged, 80 and over
• Alanine Transaminase (blood)
• Aspartate Aminotransferases (blood)
• Biomarkers (blood)
• Cytokines (blood)
• Female
• Granulocyte Colony-Stimulating Factor (blood)
• Hemostatic Techniques (adverse effects)
• Hepatectomy (adverse effects)
• Humans
• Hyaluronic Acid (blood)
• Interleukins (blood)
• Liver (blood supply)
• Macrophage Colony-Stimulating Factor (blood)
• Male
• Middle Aged
• Reperfusion Injury (diagnosis, etiology)
• Stress, Physiological (diagnosis, etiology)
• Tumor Necrosis Factor-alpha (analysis)