Antiflammin-2 prevents HL-60 adhesion to endothelial cells and prostanoid production induced by lipopolysaccharides.

Abstract

We studied the effect of antiflammin-2 (AF-2) on adhesion molecule expression by HL-60 cells and endothelial (ECV304) cells stimulated by lipopolysaccharides (LPSs), and on leukocyte-endothelial cell interaction in an in vitro coculture system. The action of AF-2 on prostanoid production in these experimental conditions was also tested. LPS increased the adhesion molecule expression, such as lymphocyte function-associated antigen-1 and membrane attack complex-1 on HL-60 cells and E-selectin and intercellular adhesion molecule-1 on ECV304 cells. The LPS-stimulated adhesion molecule expression on HL-60/ECV304 coculture system was higher than on HL-60 or ECV304 cultures. LPS also induced HL-60 adhesion to ECV304 monolayer and thromboxane B(2) and prostaglandin E(2) (PGE(2)) production in HL-60 culture and PGE(2) in ECV304 culture. Prostanoid production by HL-60/ECV304 cocultures was higher than by simple cultures. AF-2 inhibited the enhancement of adhesion molecule expression induced by LPSs, especially E-selectin. Thus, AF-2 significantly reduced the HL-60 adhesion to endothelial cells stimulated by LPSs. AF-2 also inhibited prostanoid synthesis by ECV304 cells or HL-60/ECV304 coculture challenged by LPSs. In conclusion, AF-2 reduced HL-60 adhesion to endothelial cells, suggesting that it reduces inflammation by blocking leukocyte trafficking and the subsequent eicosanoid production.

• Authors: J J Moreno
• Journal: The Journal of pharmacology and experimental therapeutics (J Pharmacol Exp Ther) Vol. 296 Issue 3 Pg. 884-9 (Mar 2001) ISSN: 0022-3565 [Print] United States
• PMID: 11181920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Cell Adhesion Molecules
• Lipopolysaccharides
• Oligopeptides
• Peptide Fragments
• antiflammin P2
• Thromboxane B2
• Dinoprostone

Topics

• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Cell Adhesion (drug effects)
• Cell Adhesion Molecules (drug effects, metabolism)
• Dinoprostone (metabolism)
• Drug Interactions
• Endothelium, Vascular (drug effects, physiology)
• HL-60 Cells
• Humans
• Lipopolysaccharides (pharmacology)
• Oligopeptides (pharmacology)
• Peptide Fragments (pharmacology)
• Thromboxane B2 (metabolism)