Inhibition of the constitutive and induced IFN-beta production by IL-4 and IL-10 in murine peritoneal macrophages.

Abstract

We had previously reported that freshly harvested peritoneal macrophages (PM) are in a type I IFN-mediated antiviral state, which is lost during in vitro culture of PM, concomitantly with a progressive decline in the expression of IFN-beta. We report herein that in vitro culture of PM in the presence of IL-4 or IL-10 results in an enhanced decay of the IFN-beta-mediated antiviral state to vesicular stomatitis virus (VSV). Moreover, IL-4 and IL-10 inhibited the production of type I IFN induced by LPS or NDV infection, as assessed by IFN production and induction of IFN-mediated antiviral state. The accumulation and physiological turnover of IFN-beta mRNA was not affected by IL-4 or IL-10. Finally, neither IL-10 nor IL-4 exerted any inhibitory effect on the antiviral activity induced by exogenous type-I IFN. These results suggest that Th2 cytokines, such as IL-4 and IL-10, act as negative regulators of the type I IFN-mediated antiviral response in PM and may represent stop signals for the constitutive or induced type I IFN expression in PM.

• Authors: B Varano, L Fantuzzi, P Puddu, P Borghi, F Belardelli, S Gessani
• Journal: Virology (Virology) Vol. 277 Issue 2 Pg. 270-7 (Nov 25 2000) ISSN: 0042-6822 [Print] United States
• PMID: 11080475 (Publication Type: Journal Article)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Lipopolysaccharides
• RNA, Messenger
• Interleukin-10
• Interleukin-4
• Interferon-beta

Topics

• Animals
• Cells, Cultured
• Dose-Response Relationship, Drug
• Down-Regulation
• Interferon-beta (biosynthesis, genetics)
• Interleukin-10 (pharmacology)
• Interleukin-4 (pharmacology)
• Lipopolysaccharides
• Macrophages, Peritoneal (drug effects, immunology, virology)
• Male
• Mice
• Mice, Inbred C3H
• Newcastle disease virus
• RNA, Messenger (genetics)
• Specific Pathogen-Free Organisms
• Time Factors
• Vesicular stomatitis Indiana virus