Ischemia-reperfusion injury in renal transplantation is independent of the immunologic background.

Abstract

BACKGROUND:

Adhesion molecule expression is important to early transplant failure. However, whether or not adhesion molecule-facilitated inflammation is antigen-dependent is unknown. We tested this hypothesis.

METHODS:

Rat renal grafts were four-hours cold-preserved in University of Wisconsin (UW) solution, transplanted to syngeneic or allogeneic recipients, and harvested after 2, 6, 12, 24, and 48 hours and after 1 week. The first allogeneic group receive no immunosuppression; two additional groups received either low (1.5 mg/kg) or standard (5 mg/kg) cyclosporine A (CsA). Renal function and morphology were determined; frozen sections were immunostained for P-selectin, L-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), platelet endothelial cell adhesion molecule-1 (PECAM-1), leukocyte function associated molecule-1 (LFA-1), very late antigen-4 (VLA-4), as well as for neutrophils and monocytes.

RESULTS:

Selectins increased rapidly at 2 hours and quickly decreased by 12 hours. While P-selectin was expressed on vasculature, L-selectin was found on inflammatory cells. Neutrophil influx and that of LFA-1-positive cells occurred early, peaked between 12 and 24 hours, and paralleled the maximal impairment in renal function. ICAM-1 and PECAM-1 showed similar kinetics and a diffuse distribution. VCAM-1 increased more slowly after 12 hours, peaked at 24 hours, and was localized predominantly on the endothelium of elastic vessels. Between 24 hours and 1 week, all grafts progressively developed dense VLA-4-positive monocytic infiltrates adjacent to vessels expressing VCAM-1. Functional, morphological, and immunohistochemical parameters did not differ between isografts and allografts at one week. However, by day 10, allografts showed severe vascular and cellular rejection, while injury in isografts resolved. Immunosuppression with CsA did not reverse the inflammation induced by ischemia-reperfusion injury.

CONCLUSIONS:

The early inflammation after ischemia-reperfusion injury is largely independent of the immunologic background. We suggest that initial injury prevention should receive the highest priority.

• Authors: D Dragun, U Hoff, J K Park, Y Qun, W Schneider, F C Luft, H Haller
• Journal: Kidney international (Kidney Int) Vol. 58 Issue 5 Pg. 2166-77 (Nov 2000) ISSN: 0085-2538 [Print] United States
• PMID: 11044238 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Adhesion Molecules
• Immunosuppressive Agents
• Integrin alpha4beta1
• Integrins
• Lymphocyte Function-Associated Antigen-1
• Receptors, Lymphocyte Homing
• Selectins
• Cyclosporine

Topics

• Animals
• Cell Adhesion Molecules (metabolism)
• Cyclosporine (pharmacology)
• Graft Rejection (pathology)
• Immunosuppressive Agents (pharmacology)
• Integrin alpha4beta1
• Integrins (metabolism)
• Ischemia (metabolism, pathology, physiopathology)
• Kidney (drug effects, metabolism, pathology)
• Kidney Transplantation (immunology)
• Lymphocyte Function-Associated Antigen-1 (metabolism)
• Male
• Necrosis
• Nephritis (etiology)
• Rats
• Rats, Inbred F344
• Rats, Inbred Lew
• Receptors, Lymphocyte Homing (metabolism)
• Renal Circulation
• Reperfusion Injury (complications, metabolism, pathology, physiopathology)
• Selectins (metabolism)
• Transplantation, Homologous
• Transplantation, Isogeneic