Suppression of N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis by dietary feeding of 1'-acetoxychavicol acetate.

Abstract

The modifying effects of 1'-acetoxychavicol acetate (ACA) on N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumorigenesis were investigated in male F344 rats. At 5 weeks of age, all test animals, except those given the test chemical alone, and the control rats received s.c. injections of NMBA (0.5 mg/kg body weight/injection, three times per week) for 5 weeks. At the termination of the study (20 weeks), 75% of rats treated with NMBA alone had esophageal neoplasms (papillomas). However, the groups given a dose of 500 ppm ACA during the initiation phase developed a significantly reduced incidence of tumors (29%; P<0.01). Exposure to ACA (500 ppm) during the post-initiation phase also decreased the frequency of the tumors (38%; P<0.05). A reduction of the incidence of preneoplastic lesions (hyperplasia or dysplasia) was obtained when ACA was administered in the initiation phase (P<0.01). Cell proliferation in the esophageal epithelium, determined by assay of proliferating cell nuclear antigen (PCNA), was lowered by ACA (P<0.05). Blood polyamine contents in rats given NMBA and the test compound were also smaller than those of rats given the carcinogen (P<0.05). These findings suggest that dietary ACA is effective in inhibiting the development of esophageal tumors by NMBA when given during the initiation or post-initiation phase, and such inhibition is related to suppression of cell proliferation in the esophageal epithelium.

• Authors: K Kawabata, T Tanaka, T Yamamoto, J Ushida, A Hara, A Murakami, K Koshimizu, H Ohigashi, G D Stoner, H Mori
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 91 Issue 2 Pg. 148-55 (Feb 2000) ISSN: 0910-5050 [Print] Japan
• PMID: 10761701 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Benzyl Alcohols
• Biogenic Polyamines
• DNA Adducts
• Proliferating Cell Nuclear Antigen
• Terpenes
• nitrosobenzylmethylamine
• Dimethylnitrosamine
• 1'-acetoxychavicol acetate

Topics

• Animals
• Anticarcinogenic Agents (pharmacology)
• Benzyl Alcohols
• Biogenic Polyamines (analysis)
• DNA Adducts (analysis)
• Dimethylnitrosamine (analogs & derivatives, toxicity)
• Esophageal Neoplasms (prevention & control)
• Male
• Precancerous Conditions (prevention & control)
• Proliferating Cell Nuclear Antigen (analysis)
• Rats
• Rats, Inbred F344
• Terpenes (pharmacology)