Abstract |
MRL/MpJ-lpr/lpr (MRL/lpr) mice spontaneously develop immune complex-mediated glomerulonephritis and thrombocytopenia. Although the presence of cross-reactive anti- phospholipid antibodies in sera of MRL/lpr mice has been demonstrated, possible relationships between detected autoantibodies and the development of thrombocytopenia have not been elucidated. Recent genetic analyses in a few different strains of lupus-prone mice have pointed out a close correlation between autoantibodies reactive with endogenous retroviral env gene product, gp70, and the development and severity of glomerulonephritis. In the process of establishing possibly nephritogenic anti-gp70 autoantibody-producing hybridoma cells from MRL/lpr mice, we identified an IgG2a-producing anti-gp70 hybridoma clone that induced microvascular intraluminal platelet aggregation, thrombocytopenia, and amenia upon transplantation into syngeneic non-autoimmune mice. This and two other anti-gp70 antibodies bound onto the surface of mouse platelets, and purified IgG2a of the anti-gp70 autoantibody induced glomerular lesions with characteristics of thrombotic thrombocytopenic purpura when injected into non-autoimmune mice. The pathogenic anti-gp70 autoantibody specifically precipitated a platelet protein with an approximate relative molecular mass of 40 000.
|
Authors | K Hashimoto, N Tabata, R Fujisawa, H Matsumura, M Miyazawa |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 119
Issue 1
Pg. 47-56
(Jan 2000)
ISSN: 0009-9104 [Print] England |
PMID | 10606963
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Monoclonal
- Autoantibodies
- Immunoglobulin G
- Viral Envelope Proteins
|
Topics |
- Animals
- Antibodies, Monoclonal
(administration & dosage)
- Autoantibodies
(administration & dosage)
- Blood Platelets
(immunology)
- Cell Line
- Female
- Friend murine leukemia virus
(immunology)
- Glomerulonephritis
(etiology, immunology, pathology)
- Hybridomas
(immunology)
- Immunization, Passive
- Immunoglobulin G
(administration & dosage)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred MRL lpr
- Platelet Aggregation
(immunology)
- Purpura, Thrombotic Thrombocytopenic
(etiology, immunology, pathology)
- Viral Envelope Proteins
(immunology)
|