Detection of codon 12 mutation in the K-ras oncogene in pancreatic tumors.

Abstract

Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.

• Authors: M S Kubrusly, E Matheucci Júnior, K R Leite, A M Coelho, O Monte, M C Machado, H W Pinotti
• Journal: Revista do Hospital das Clinicas (Rev Hosp Clin Fac Med Sao Paulo) 1999 Jan-Feb Vol. 54 Issue 1 Pg. 17-20 ISSN: 0041-8781 [Print] Brazil
• PMID: 10488596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Codon
• DNA, Neoplasm

Topics

• Codon (analysis)
• DNA, Neoplasm (analysis, isolation & purification)
• Genes, ras (genetics)
• Humans
• Mutation (genetics)
• Pancreatic Neoplasms (genetics)
• Sensitivity and Specificity