Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome.

Abstract

Odontogenic keratocysts are occasionally (4-5%) associated with the nevoid basal cell carcinoma syndrome, a pleiotropic, autosomal disorder presenting a spectrum of developmental abnormalities and a predisposition for the development of different neoplasms. The aim of this study was to establish whether keratocysts showing clinically aggressive behavior associated with nevoid basal cell carcinoma syndrome reflect differences in cellular proliferation rate and/or in the expression of oncoproteins and tumor suppressor genes. For this reason, formalin-fixed paraffin-embedded sections of odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome (16 cases) and sporadic odontogenic keratocysts (16 cases) were compared for expression of proliferating cell nuclear antigen (PCNA) and p53, bcl-2, and bcl-1 (cyclin D1) onco-proteins. Most of the epithelial lining of odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome showed nuclear immunopositivity for p53 protein and overexpression of cyclin D1 with various degrees of staining intensity. All sporadic odontogenic keratocysts were negative for p53 and cyclin D1. The expressions of bcl-2 oncoprotein were found to be substantially similar between the two groups of lesions, with a cytoplasmic immunopositivity localized only in the resting reserve basal layer of the epithelium. PCNA expression showed no statistically significant difference between the two groups of lesions. In conclusion, the finding of cyclin D1 and p53 overexpression in odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome could be considered a hallmark of a mutated cellular phenotype, thus leading to the hypothesis that their aggressive clinical behavior could be due to a dysregulation of the expression of cyclin D1 and p53 proteins, involved in a check-point control of cellular proliferation.

• Authors: L Lo Muzio, S Staibano, G Pannone, P Bucci, P F Nocini, E Bucci, G De Rosa
• Journal: Journal of dental research (J Dent Res) Vol. 78 Issue 7 Pg. 1345-53 (Jul 1999) ISSN: 0022-0345 [Print] United States
• PMID: 10403462 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oncogene Proteins
• Proliferating Cell Nuclear Antigen
• Proto-Oncogene Proteins c-bcl-2
• Tumor Suppressor Protein p53
• Cyclin D1

Topics

• Analysis of Variance
• Apoptosis (genetics)
• Basal Cell Nevus Syndrome (genetics, pathology)
• Cell Cycle (genetics)
• Cell Division (genetics)
• Cell Nucleus (ultrastructure)
• Cyclin D1 (analysis, genetics)
• Cytoplasm (ultrastructure)
• Epithelial Cells (pathology)
• Epithelium (pathology)
• Female
• Gene Expression Regulation
• Gene Expression Regulation, Neoplastic
• Genes, Tumor Suppressor (genetics)
• Genetic Predisposition to Disease
• Humans
• Immunohistochemistry
• Male
• Mutation (genetics)
• Odontogenic Cysts (genetics, pathology)
• Oncogene Proteins (genetics)
• Phenotype
• Proliferating Cell Nuclear Antigen (analysis, genetics)
• Proto-Oncogene Proteins c-bcl-2 (analysis, genetics)
• Tumor Suppressor Protein p53 (analysis, genetics)