The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo.

Abstract

BACKGROUND:

Jawoongo is an herbal mixture used in traditional medicine to treat skin diseases. This study aimed to investigate whether Jawoongo ameliorates Atopic dermatitis (AD)-like pathology in mice and to understand its underlying cellular mechanisms.

METHODS:

AD was induced by 2, 4-Dinitrocholrlbenzene (DNCB) in BALB/c mice. Treatment with Jawoongo was assessed to study the effect of Jawoongo on AD in mice. Histological Analysis, blood analysis, RT-PCR, western blot analysis, ELISA assay and cell viability assay were performed to verify the inhibitory effect of Jawoongo on AD in mice.

RESULTS:

We found that application of Jawoongo in an ointment form on AD-like skin lesions on DNCB-exposed BALB/c mice reduced skin thickness and ameliorated skin infiltration with inflammatory cells, mast cells and CD4+ cells. The ointment also reduced the mRNA levels of IL-2, IL-4, IL-13 and TNF-α in the sensitized skin. Leukocyte counts and the levels of IgE, IL-6, IL-10 and IL-12 were decreased in the blood of the DNCB-treated mice. Furthermore, studies on cultured cells demonstrated that Jawoongo exhibits anti-inflammatory activities, including the suppression of proinflammatory cytokine expression, nitric oxide (NO) production, and inflammation-associated molecule levels in numerous types of agonist-stimulated innate immune cell, including human mast cells (HMC-1), murine macrophage RAW264.7 cells, and splenocytes isolated from mice.

CONCLUSION:

These findings indicate that Jawoongo alleviates DNCB-induced AD-like symptoms via the modulation of several inflammatory responses, indicating that Jawoongo might be a useful drug for the treatment of AD.

• Authors: Jin Mo Ku, Se Hyang Hong, Soon Re Kim, Han-Seok Choi, Hyo In Kim, Dong Uk Kim, So Mi Oh, Hye Sook Seo, Tai Young Kim, Yong Cheol Shin, Chunhoo Cheon, Seong-Gyu Ko
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 18 Issue 1 Pg. 215 (Jul 13 2018) ISSN: 1472-6882 [Electronic] England
• PMID: 30005655 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Dinitrochlorobenzene
• Interleukin-13
• Interleukin-6
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Immunoglobulin E

Topics

• Angelica (chemistry)
• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Dermatitis, Atopic (chemically induced, drug therapy, genetics, immunology)
• Dinitrochlorobenzene (toxicity)
• Humans
• Immunoglobulin E (immunology)
• Interleukin-13 (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Lithospermum (chemistry)
• Macrophages (drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Plant Extracts (administration & dosage)
• RAW 264.7 Cells
• Tumor Necrosis Factor-alpha (genetics, immunology)