Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: Although CD9 was detected in the epithelium of normal prostatic tissues, reduced or loss of CD9 expression within neoplastic cells was observed in 24% of 107 clinically localized primary adenocarcinomas, 85% of 60 clinically advanced primary adenocarcinomas, 85% of 65 lymph node metastases, and 65% of 23 bone metastases. Difference in CD9 expression between clinically localized and advanced diseases was highly significant (P < 1 x 10(-7)). Whereas there was no alteration of CD9 cDNA in normal tissues, all PC-3-derived cell lines, one PIN, and four prostatic adenocarcinomas harbored deletions in their CD9 cDNAs. Recurring CD9 point mutations were also found in PC-3M-LN4 cells, one PIN, and seven prostatic adenocarcinomas. CONCLUSIONS: CD9 expression is significantly reduced and even lost during prostate cancer progression. Moreover, deletions and mutations of the CD9 mRNA may be associated with loss of protein expression observed in tumor cells. Our data suggest that CD9 inactivation may play an important role in prostate cancer progression.
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Authors | Jia-Chi Wang, Louis R Bégin, Nathalie G Bérubé, Simone Chevalier, Armen G Aprikian, Henriette Gourdeau, Mario Chevrette |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 13
Issue 8
Pg. 2354-61
(Apr 15 2007)
ISSN: 1078-0432 [Print] United States |
PMID | 17406028
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- CD9 protein, human
- DNA, Complementary
- DNA, Neoplasm
- Membrane Glycoproteins
- RNA, Messenger
- RNA, Neoplasm
- Tetraspanin 29
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Topics |
- Adenocarcinoma
(genetics, immunology, pathology)
- Antigens, CD
(genetics)
- Cell Line, Tumor
- Cloning, Molecular
- DNA, Complementary
(genetics)
- DNA, Neoplasm
(genetics)
- Disease Progression
- Gene Deletion
- Gene Expression Regulation, Neoplastic
- Gene Silencing
- Humans
- Male
- Membrane Glycoproteins
(genetics)
- Prostatic Neoplasms
(genetics, immunology, pathology)
- RNA, Messenger
(genetics)
- RNA, Neoplasm
(genetics)
- Tetraspanin 29
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