Priming of T-cell responses in mice by porins of Salmonella typhimurium.

Abstract

An imbalance in signals delivered to T cells via T-cell receptor and accessory molecules can lead to anergy, apoptosis, or both. In the present study we have demonstrated that Salmonella typhimurium infection in mice leads to a progressive loss of CD4+ T helper (Th) cell population, abnormal T-cell death by apoptosis and loss of accessory molecules (B7 and intracellular adhesion molecule-1) on macrophages. Quantification of interleukin-2 (IL-2), interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) secretion revealed a Th2-type of response in lymphocytes isolated from spleen. However, preimmunization of mice with porins resulted in an increased CD4+ Th cell population and accessory molecules on the surface of macrophages. Quantification of cytokines revealed a Th1-type of response. We conclude that preimmunization of mice with porins provides a microenvironment in which a well-balanced accessory molecule and cytokine network is established, which results in the prevention of cell death by apoptosis.

• Authors: S Gupta
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 48 Issue 2 Pg. 136-43 (Aug 1998) ISSN: 0300-9475 [Print] England
• PMID: 9716104 (Publication Type: Journal Article)

Chemical References

• B7-1 Antigen
• Interleukins
• Porins
• Intercellular Adhesion Molecule-1
• Interferon-gamma

Topics

• Animals
• Apoptosis
• B7-1 Antigen (metabolism)
• CD4-Positive T-Lymphocytes (immunology)
• Flow Cytometry
• Immune Tolerance
• Immunization
• Intercellular Adhesion Molecule-1 (metabolism)
• Interferon-gamma (metabolism)
• Interleukins (metabolism)
• Macrophages (immunology)
• Mice
• Mice, Inbred BALB C
• Porins (immunology, pharmacology)
• Salmonella Infections, Animal (immunology, prevention & control)
• Salmonella typhi (metabolism)